Zhang Xuanming, An Dongqing, Guo Longlong
College of TCM, Xinjiang Medical University, Urumqi, Xinjiang, China/Department of TCM, the First Affiliated Hospital of the Medical College, Shihezi University, Shihezi, China.
College of TCM, Xinjiang Medical University, Urumqi, Xinjiang, China.
Pak J Pharm Sci. 2019 Nov;32(6):2611-2615.
Ziziphora clinopodioide Lam, a traditional Chinese medicinal plant, has been used to treat hypertension, coronary heart disease and other cardiovascular diseases, autophagy plays an important role in these diseases. This study investigated the effects of Z. clinopodioides and its active components on autophagy using cell biology. Normal rat kidney (NRK) cells transfected with green fluorescent protein-associated microtubule-protein 1 light Chain 3 (GFP-LC3) were intervened with different doses of ethanol and water extracts of Z. clinopodioides and the active components of Z. clinopodioides. After 4 hours treatment, the autophagy spot aggregation in NRK cells was photographed and observed by laser scanning confocal microscopy. The results showed that the water and ethanol extracts of Z. clinopodioides can activate autophagy, the effect of activating autophagy was more significant, when the dose was increased. Five components including chrysin, luteolin, quercetin, oleanolic acid and ursolic acid were identified as the active principles in activating autophagy. This research may provide a reference for the further study of mechanism and material basis of Z. clinopodioides in treatment of cardiovascular diseases.
风轮菜是一种传统的中药材，已被用于治疗高血压、冠心病等心血管疾病，自噬在这些疾病中起着重要作用。本研究采用细胞生物学方法，研究了风轮菜及其活性成分对自噬的影响。用绿色荧光蛋白相关微管蛋白1轻链3（GFP-LC3）转染的正常大鼠肾（NRK）细胞，分别用不同剂量的风轮菜乙醇提取物、水提取物及其活性成分进行干预。处理4小时后，通过激光扫描共聚焦显微镜对NRK细胞中的自噬斑点聚集情况进行拍照和观察。结果表明，风轮菜的水提取物和乙醇提取物均可激活自噬，且随着剂量增加，激活自噬的效果更显著。白杨素、木犀草素、槲皮素、齐墩果酸和熊果酸5种成分被确定为激活自噬的活性成分。本研究可为进一步研究风轮菜治疗心血管疾病的作用机制和物质基础提供参考。
