Research Conducted at Department of Chemistry, King's College London, London, United Kingdom.
Research Conducted at Department of Chemistry, King's College London, London, United Kingdom.
Bioorg Med Chem. 2018 Oct 15;26(19):5253-5258. doi: 10.1016/j.bmc.2018.03.036. Epub 2018 Mar 26.
Methods for the generation of nanoparticles encapsulated within cage proteins, such as ferritins, provide particles with low polydispersities due to size constraint by the cage. The proteins can provide enhanced water solubility to enable biological applications and affinity and identification tags to facilitate delivery or the assembly of advanced materials. Many effective methods have been developed, however, they are often impeded by cage protein instability in the presence of reagents or conditions for formation of the nanoparticles. Although the stability of ferritin cage quaternary structure can be enhanced, application of ferritins to materials science remains limited by unpredictable behaviour. Recently, we reported a medium throughput technique to directly detect the ferritin cage state. Herein, we expand this strategy to screen conditions commonly used for the formation of gold nanoparticles. Not only do we report nanoparticle formation conditions that permit ferritin stability, we establish a general screening strategy based on protein cage stability that could be applied to other protein cages or for the generation of other types of particles.
笼蛋白（如铁蛋白）内包裹纳米颗粒的生成方法提供了低多分散性的颗粒，因为颗粒大小受到笼的限制。该蛋白质可提高水溶解度，以实现生物应用，并提供亲和性和识别标记，以促进递药或高级材料的组装。已经开发了许多有效的方法，然而，由于试剂的存在或纳米颗粒形成的条件，笼蛋白的不稳定性常常阻碍了这些方法的应用。尽管铁蛋白笼四级结构的稳定性可以得到增强，但铁蛋白在材料科学中的应用仍然受到不可预测行为的限制。最近，我们报道了一种高通量技术，可以直接检测铁蛋白笼的状态。在这里，我们扩展了这一策略，以筛选通常用于金纳米颗粒形成的条件。我们不仅报告了允许铁蛋白稳定的纳米颗粒形成条件，而且还建立了一种基于蛋白质笼稳定性的通用筛选策略，该策略可应用于其他蛋白质笼或用于生成其他类型的颗粒。
