Mayyas F, Jaradat R, Alzoubi K H
Department of Clinical Pharmacy, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, Jordan.
Department of Clinical Pharmacy, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, Jordan.
Nutr Metab Cardiovasc Dis. 2018 Jun;28(6):592-599. doi: 10.1016/j.numecd.2018.02.012. Epub 2018 Feb 25.
Fish oil (FO) is rich in omega-3 polyunsaturated fatty acids, which have cardio-protective effects. This study aims to evaluate effects of FO in a rat model of streptozotocin (STZ) induced diabetes.
Adults male Wistar rats were assigned to control (4 μl corn oil/g corn oil given by oral gavage), FO (4 μl Menhaden FO/g body weight given by oral gavage), diabetes (DM, 35 mg/kg STZ single intraperitoneal injection, corn oil), and DM + FO groups for 8 weeks. Plasma and cardiac biomarkers of oxidative stress, inflammation, and fibrosis were evaluated. STZ-induced diabetes as indicated by the significant increase in serum levels of glucose and percentage of glycated hemoglobins. FO reduced plasma arachidonic acid (AA) percentage and ratio of AA: docosahexaenoic acid (DHA). Plasma and cardiac levels of total nitrite, endothelin -1 (ET-1), and myeloperoxidase (MPO) increased in the DM group, whereas cardiac activities of catalase and superoxide dismutase (SOD) decreased. FO reduced cardiac nitrite and MPO, and plasma ET-1 levels. FO increased cardiac glutathione, catalase and SOD activities. Levels of thiobarbituric acid substances increased in the FO and DM groups with significant synergism in the DM + FO group. FO prevented cardiac fibrosis associated with DM and decreased cardiac transforming growth factor beta-1and p38 MAP kinases. Cardiac levels of matrix metalloproteinase -2 were significantly elevated in FO and DM + FO groups.
FO decreased plasma and cardiac oxidative stress, inflammation and myocardial fibrosis. FO could be used in diabetes to reduce risk and burden of CVDs.
鱼油（FO）富含ω-3多不饱和脂肪酸，具有心脏保护作用。本研究旨在评估鱼油对链脲佐菌素（STZ）诱导的糖尿病大鼠模型的影响。
将成年雄性Wistar大鼠分为对照组（经口灌胃给予4μl玉米油/克体重玉米油）、鱼油组（经口灌胃给予4μl鲱鱼油/克体重）、糖尿病组（DM，单次腹腔注射35mg/kg STZ，玉米油）和糖尿病+鱼油组，持续8周。评估血浆和心脏中氧化应激、炎症和纤维化的生物标志物。STZ诱导的糖尿病表现为血清葡萄糖水平和糖化血红蛋白百分比显著升高。鱼油降低了血浆花生四烯酸（AA）百分比以及AA与二十二碳六烯酸（DHA）的比例。糖尿病组血浆和心脏中的总亚硝酸盐、内皮素-1（ET-1）和髓过氧化物酶（MPO）水平升高，而心脏中的过氧化氢酶和超氧化物歧化酶（SOD）活性降低。鱼油降低了心脏亚硝酸盐和MPO水平以及血浆ET-1水平。鱼油增加了心脏谷胱甘肽、过氧化氢酶和SOD活性。鱼油组和糖尿病组中硫代巴比妥酸物质水平升高，糖尿病+鱼油组具有显著协同作用。鱼油预防了与糖尿病相关的心脏纤维化，并降低了心脏转化生长因子β-1和p38丝裂原活化蛋白激酶水平。鱼油组和糖尿病+鱼油组心脏基质金属蛋白酶-2水平显著升高。
鱼油降低了血浆和心脏的氧化应激、炎症以及心肌纤维化。鱼油可用于糖尿病患者以降低心血管疾病的风险和负担。
