Bergmann Katharina, Meza Santoscoy Paola, Lygdas Konstantinos, Nikolaeva Yulia, MacDonald Ryan B, Cunliffe Vincent T, Nikolaev Anton
Department of Biomedical Science, The University of Sheffield, Western Bank, Sheffield S10 2TN, UK.
Department of Infection, Immunity and Cardiovascular Disease, The University of Sheffield, Western Bank Sheffield, S10 2TN, UK.
J Dev Biol. 2018 Mar 9;6(1):6. doi: 10.3390/jdb6010006.
The zebrafish is an established model to study the development and function of visual neuronal circuits in vivo, largely due to their optical accessibility at embryonic and larval stages. In the past decade multiple experimental paradigms have been developed to study visually-driven behaviours, particularly those regulated by the optic tectum, the main visual centre in lower vertebrates. With few exceptions these techniques are limited to young larvae (7-9 days post-fertilisation, dpf). However, many forms of visually-driven behaviour, such as shoaling, emerge at later developmental stages. Consequently, there is a need for an experimental paradigm to image the visual system in zebrafish larvae beyond 9 dpf. Here, we show that using line allows for imaging neuronal activity in the optic tectum in late stage larvae until at least 21 dpf. Utilising this line, we have characterised the receptive field properties of tectal neurons of the 2-3 weeks old fish in the cell bodies and the neuropil. line provides a complementary approach and additional opportunities to study neuronal activity in late stage zebrafish larvae.
斑马鱼是研究视觉神经元回路在体内发育和功能的成熟模型，这主要归功于其在胚胎和幼体阶段的光学可及性。在过去十年中，已经开发了多种实验范式来研究视觉驱动行为，特别是那些由视顶盖（低等脊椎动物的主要视觉中枢）调节的行为。除了少数例外，这些技术仅限于幼体（受精后7 - 9天，dpf）。然而，许多形式的视觉驱动行为，如集群行为，在发育后期才出现。因此，需要一种实验范式来对9 dpf以上的斑马鱼幼体的视觉系统进行成像。在这里，我们表明使用 系可以对晚期幼体视顶盖中的神经元活动进行成像，直至至少21 dpf。利用该品系，我们已经在细胞体和神经纤维网中表征了2 - 3周龄鱼类视顶盖神经元的感受野特性。 系为研究晚期斑马鱼幼体的神经元活动提供了一种补充方法和额外机会。
