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从柑橘青霉中鉴定出两种新型真菌病毒及其与相关杀菌剂抗性的分析。

Characterization of two novel mycoviruses from Penicillium digitatum and the related fungicide resistance analysis.

机构信息

Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, P. R. China.

出版信息

Sci Rep. 2018 Apr 3;8(1):5513. doi: 10.1038/s41598-018-23807-3.

DOI:10.1038/s41598-018-23807-3
PMID:29615698
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5882929/
Abstract

Pathogenic fungi including Penicillium digitatum and Penicillium italicum are the main destructive pathogens in the citrus industry, causing great losses during postharvest process. To our knowledge, only one mycovirus from P. digitatum has been reported, and the prevalence of such mycoviruses against citrus postharvest pathogenic fungi and their genotyping were still under investigation. In the present study, we showed that 39 of 152 Penicillium isolates from main citrus-growing areas in China were infected with various mycoviruses belonging to polymycoviruses, Narna-like viruses, and families Totiviridae, Partitivirdae and Chrysoviridae. The next generation sequencing (NGS) towards virus genome library and the following molecular analysis revealed two novel mycoviruses Penicillium digitatum polymycovirus 1 (PdPmV1) and Penicillium digitatum Narna-like virus 1 (PdNLV1), coexisting in P. digitatum strain HS-RH2. The fungicide-resistant P. digitatum strains HS-F6 and HS-E9 coinfected by PdPmV1 and PdNLV1 exhibited obvious reduction in triazole drug prochloraz resistance by mycelial growth analysis on both PDA plates and citrus fruit epidermis with given prochloraz concentration. This report at the first time characterized two novel mycoviruses from P. digitatum and revealed the mycovirus-induced reduction of fungicide resistance.

摘要

包括青霉属（Penicillium digitatum 和 Penicillium italicum）在内的病原真菌是柑橘产业中主要的破坏性病原物，在采后过程中造成了巨大损失。据我们所知，仅从青霉属（Penicillium digitatum）中报道过一种真菌病毒，而针对柑橘采后病原真菌的此类真菌病毒的流行情况及其基因型仍在调查中。在本研究中，我们表明，来自中国主要柑橘种植区的 152 个青霉属分离物中有 39 个感染了属于多瘤病毒科、Narna-like 病毒科以及 totiviridae、partitiviridae 和 chrysoviridae 科的各种真菌病毒。针对病毒基因组文库的下一代测序（NGS）和随后的分子分析揭示了两种新的真菌病毒，即青霉属（Penicillium digitatum）多瘤病毒 1（PdPmV1）和青霉属（Penicillium digitatum）Narna-like 病毒 1（PdNLV1），它们共存于青霉属（Penicillium digitatum）菌株 HS-RH2 中。对同时感染 PdPmV1 和 PdNLV1 的抗真菌剂青霉属（Penicillium digitatum）菌株 HS-F6 和 HS-E9 进行的菌丝生长分析以及在给定的丙环唑浓度下在 PDA 平板和柑橘果皮上的分析表明，丙环唑抗性明显降低。本报告首次从青霉属（Penicillium digitatum）中鉴定出两种新的真菌病毒，并揭示了真菌病毒诱导降低抗真菌剂抗性的现象。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/655c/5882929/7b41dd480358/41598_2018_23807_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/655c/5882929/f43817a7644a/41598_2018_23807_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/655c/5882929/5c20c03a6cb9/41598_2018_23807_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/655c/5882929/58abc3e5e1bc/41598_2018_23807_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/655c/5882929/1bb7c1e78861/41598_2018_23807_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/655c/5882929/7b41dd480358/41598_2018_23807_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/655c/5882929/f43817a7644a/41598_2018_23807_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/655c/5882929/5c20c03a6cb9/41598_2018_23807_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/655c/5882929/58abc3e5e1bc/41598_2018_23807_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/655c/5882929/1bb7c1e78861/41598_2018_23807_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/655c/5882929/7b41dd480358/41598_2018_23807_Fig5_HTML.jpg

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