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电子烟和烟草烟雾暴露对慢性阻塞性肺疾病支气管上皮细胞炎症反应的影响。

The effect of electronic cigarette and tobacco smoke exposure on COPD bronchial epithelial cell inflammatory responses.

作者信息

Higham Andrew, Bostock Declan, Booth George, Dungwa Josiah V, Singh Dave

机构信息

Division of Infection, Immunity and Respiratory Medicine, School of Biological Sciences, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, The University of Manchester and University Hospital of South Manchester, NHS Foundation Trust, Manchester, UK.

Medicines Evaluation Unit, University Hospital of South Manchester, Manchester, UK.

出版信息

Int J Chron Obstruct Pulmon Dis. 2018 Mar 23;13:989-1000. doi: 10.2147/COPD.S157728. eCollection 2018.

DOI:10.2147/COPD.S157728
PMID:29615835
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5870631/
Abstract

BACKGROUND

Electronic cigarettes (e-cigs) are used to help smoking cessation. However, these devices contain harmful chemicals, and there are safety concerns. We have investigated the effects of e-cigs on the inflammatory response and viability of COPD bronchial epithelial cells (BECs).

METHODS

BECs from COPD patients and controls were exposed to e-cig vapor extract (ECVE) and the levels of interleukin (IL)-6, C-X-C motif ligand 8 (CXCL8), and lactate dehydrogenase release were measured. We also examined the effect of ECVE pretreatment on polyinosinic:polycytidylic acid (poly I:C)-stimulated cytokine release from BECs. Parallel experiments using Calu-3 cells were performed. Comparisons were made with cigarette smoke extract (CSE).

RESULTS

ECVE and CSE caused an increase in the release of IL-6 and CXCL8 from Calu-3 cells. ECVE only caused toxicity in BECs and Calu-3 cells. Furthermore, ECVE and CSE dampened poly I:C-stimulated C-X-C motif ligand 10 release from both cell culture models, reaching statistical significance for CSE at an optical density of 0.3.

CONCLUSION

ECVE caused toxicity and reduced the antiviral response to poly I:C. This raises concerns over the safety of e-cig use.

摘要

背景

电子烟用于帮助戒烟。然而，这些装置含有有害化学物质，存在安全问题。我们研究了电子烟对慢性阻塞性肺疾病（COPD）支气管上皮细胞（BECs）炎症反应和活力的影响。

方法

将COPD患者和对照者的BECs暴露于电子烟蒸汽提取物（ECVE），测量白细胞介素（IL）-6、C-X-C基序配体8（CXCL8）水平以及乳酸脱氢酶释放量。我们还检测了ECVE预处理对聚肌苷酸:聚胞苷酸（poly I:C）刺激的BECs细胞因子释放的影响。使用Calu-3细胞进行了平行实验。与香烟烟雾提取物（CSE）进行了比较。

结果

ECVE和CSE导致Calu-3细胞中IL-6和CXCL8释放增加。ECVE仅对BECs和Calu-3细胞产生毒性。此外，ECVE和CSE均抑制了两种细胞培养模型中poly I:C刺激的C-X-C基序配体10释放，在光密度为0.3时，CSE达到统计学显著性。

结论

ECVE导致毒性并降低了对poly I:C的抗病毒反应。这引发了对使用电子烟安全性的担忧。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae11/5870631/29fe77b5554f/copd-13-989Fig9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae11/5870631/6b2001bd95fa/copd-13-989Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae11/5870631/a4b34671d849/copd-13-989Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae11/5870631/685a7ffc0301/copd-13-989Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae11/5870631/ea94f3c59a7d/copd-13-989Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae11/5870631/d975b9ef63ec/copd-13-989Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae11/5870631/7737ecd6516f/copd-13-989Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae11/5870631/fecd0f606b4d/copd-13-989Fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae11/5870631/8a2996d5c904/copd-13-989Fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae11/5870631/29fe77b5554f/copd-13-989Fig9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae11/5870631/6b2001bd95fa/copd-13-989Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae11/5870631/a4b34671d849/copd-13-989Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae11/5870631/685a7ffc0301/copd-13-989Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae11/5870631/ea94f3c59a7d/copd-13-989Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae11/5870631/d975b9ef63ec/copd-13-989Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae11/5870631/7737ecd6516f/copd-13-989Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae11/5870631/fecd0f606b4d/copd-13-989Fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae11/5870631/8a2996d5c904/copd-13-989Fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae11/5870631/29fe77b5554f/copd-13-989Fig9.jpg

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