Erukainure Ochuko L, Ashraf Nadia, Naqvi Asma S, Zaruwa Moses Z, Muhammad Aliyu, Odusote Adenike D, Elemo Gloria N
Nutrition and Toxicology Division, Federal Institute of Industrial Research Oshodi, Lagos, Nigeria.
Faculty of Pharmacy, Barrett Hodgson University, Karachi, Pakistan.
Front Pharmacol. 2018 Mar 20;9:251. doi: 10.3389/fphar.2018.00251. eCollection 2018.
Glioblastoma multiforme (GBM) is a malignant primary type of brain cancer with high proliferation and metastasis rates due to involvement of the microglial cell. It is resistant against available chemotherapy. Many strategic protocols have been developed but prognosis and patient life has not improved substantially. In this study, the anti-metastatic and antioxidant effect of fatty acids from leaves were investigated in U87-MG (Human Glioblastoma Multiforme) cell lines. The extracted fatty acids were incubated with U87-MG cells for 48 h. The anti-proliferative effect was determined by MTT assay, while apoptosis and cell cycle were analyzed with BD FACSCalibur. The transwell assay protocol was utilized in the analysis of cell migration and invasion. The treated cell lines were also assessed for reduced glutathione (GSH) level, catalase, superoxide dismutase (SOD) and lipid peroxidation. The fatty acid extract showed significant inhibitory activity on cell proliferation and cell cycle progression, mitigated oxidative stress, and suppressed migration and invasion in U-87 MG cell lines. These results give credence to the therapeutic potential of this plant against cancer, especially GBM.
多形性胶质母细胞瘤（GBM）是一种恶性原发性脑癌，由于小胶质细胞的参与，其增殖和转移率很高。它对现有的化疗具有抗性。已经制定了许多战略方案，但预后和患者寿命并未得到实质性改善。在本研究中，研究了来自树叶的脂肪酸对U87-MG（人多形性胶质母细胞瘤）细胞系的抗转移和抗氧化作用。将提取的脂肪酸与U87-MG细胞孵育48小时。通过MTT法测定抗增殖作用，同时用BD FACSCalibur分析细胞凋亡和细胞周期。采用Transwell分析方案分析细胞迁移和侵袭。还评估了处理后的细胞系的还原型谷胱甘肽（GSH）水平、过氧化氢酶、超氧化物歧化酶（SOD）和脂质过氧化。脂肪酸提取物对U-87 MG细胞系的细胞增殖和细胞周期进程具有显著的抑制活性，减轻了氧化应激，并抑制了迁移和侵袭。这些结果证实了这种植物对抗癌症，尤其是GBM的治疗潜力。
