Aragón J J, Gómez M E, Gancedo C
Depto de Bioquímica, Facultad de Medicina UAM, Madrid, Spain.
FEBS Lett. 1987 Dec 21;226(1):121-4. doi: 10.1016/0014-5793(87)80563-x.
Two forms of 6-phosphofructo-2-kinase have been identified in Saccharomyces cerevisiae by their different chromatographic behaviour on CM-Sephadex C-50. One of them was not adsorbed and represented approximately 30% of the eluted activity. The other one emerged at about 120 mM KCl. A molecular mass of 120 kDa was found for both of them. No differences in kinetic behaviour in susceptibility to activation by cAMP-dependent protein kinase were found between the two forms.
通过6-磷酸果糖-2-激酶在CM-葡聚糖凝胶C-50上不同的色谱行为，在酿酒酵母中鉴定出了两种形式。其中一种不被吸附，约占洗脱活性的30%。另一种在约120 mM氯化钾时出现。两种形式的分子量均为120 kDa。两种形式在对环磷酸腺苷依赖性蛋白激酶激活的敏感性方面，动力学行为没有差异。
