Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China.
Inspection & Quarantine Technology Center, Fujian Entry-Exit Inspection and Quarantine Bureau, Fuzhou 350003, China.
J Zhejiang Univ Sci B. 2018;19(4):274-284. doi: 10.1631/jzus.B1700171.
We report the recovery of a 7068-nt viral sequence from the "viral fossils" embedded in the genome of Alhagi sparsifolia, a typical desert plant. Although the full viral genome remains to be completed, the putative genome structure, the deduced amino acids and phylogenetic analysis unambiguously demonstrate that this viral sequence represents a novel species of the genus Badnavirus. The putative virus is tentatively termed Alhagi bacilliform virus (ABV). Southern blotting and inverse polymerase chain reaction (PCR) data indicate that the ABV-related sequence is integrated into the A. sparsifolia genome, and probably does not give rise to functional episomal virus. Molecular evidence that the ABV sequence exists widely in A. sparsifolia is also presented. To our knowledge, this is the first endogenous badnavirus identified from plants in the Gobi desert, and may provide new clues on the evolution, geographical distribution as well as the host range of the badnaviruses.
我们从典型沙漠植物骆驼刺基因组中“病毒化石”中回收了一段 7068 个核苷酸的病毒序列。尽管完整的病毒基因组尚未完成，但推测的基因组结构、推导的氨基酸序列和系统发育分析明确表明，该病毒序列代表了伴生病毒属的一个新种。该假定的病毒暂时被命名为骆驼刺杆状病毒（ABV）。Southern 印迹和反向聚合酶链反应（PCR）数据表明，ABV 相关序列已整合到骆驼刺基因组中，并且可能不会产生有功能的游离病毒。还提供了 ABV 序列广泛存在于骆驼刺中的分子证据。据我们所知，这是首次从戈壁沙漠的植物中鉴定出内源性伴生病毒，这可能为伴生病毒的进化、地理分布以及宿主范围提供新的线索。
