MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry , Sun Yat-Sen University , Guangzhou 510275 , China.
School of Materials Science and Engineering , University of Jinan , Jinan 250022 Shandong , China.
ACS Appl Mater Interfaces. 2018 Apr 25;10(16):13264-13273. doi: 10.1021/acsami.7b12521. Epub 2018 Apr 16.
Studies on the development of fluorescent organic molecules with different emission colors for imaging of organelles and their biomedical application are gaining lots of focus recently. Here, we report two cationic organochalcogens 1 and 2, both of which exhibit very weak green emission (Φ = 0.12%; Φ = 0.09%) in dilute solution as monomers, but remarkably enhanced green emission upon interaction with nucleic acids and large red-shifted emission in aggregate state by the formation of excimers at high concentration. More interestingly, the monomer emission and excimer-like emission can be used for dual color imaging of different organelles. Upon passively diffusing into cells, both probes selectively stain nucleoli with strong green emission upon 488 nm excitation, whereas upon 405 nm excitation, a completely different stain pattern by staining lysosomes (for 1) or mitochondria (for 2) with distinct red emission is observed because of the highly concentrated accumulation in these organelles. Studies on the mechanism of the accumulation in lysosomes (for 1) or mitochondria (for 2) found that the accumulations of the probes are dependent on the membrane permeabilization, which make the probes have great potential in diagnosing cell damage by sensing lysosomal or mitochondrial membrane permeabilization. The study is demonstrative, for the first time, of two cationic molecules for dual-color imaging nucleoli and lysosomes (1)/mitochondria (2) simultaneously in live cell based on monomer and excimer-like emission, respectively, and more importantly, for diagnosing cell damage.
最近，研究具有不同发射颜色的荧光有机分子用于细胞器成像及其生物医学应用的工作受到了广泛关注。在这里，我们报告了两种阳离子有机硫属元素化合物 1 和 2，它们在稀溶液中作为单体时均显示非常弱的绿色发射（Φ=0.12%；Φ=0.09%），但与核酸相互作用时会显著增强绿色发射，并且在高浓度时通过形成激基复合物而在聚集态下发生显著的红移发射。更有趣的是，单体发射和激基类似发射可用于不同细胞器的双色成像。探针通过被动扩散进入细胞后，两种探针均选择性地在 488nm 激发下对核仁进行强绿色染色，而在 405nm 激发下，由于在这些细胞器中高度浓缩积累，观察到通过用不同的红色发射进行溶酶体（对于 1）或线粒体（对于 2）染色的完全不同的染色模式。对在溶酶体（对于 1）或线粒体（对于 2）中积累的机制的研究发现，探针的积累依赖于膜通透性，这使得探针在通过检测溶酶体或线粒体膜通透性来诊断细胞损伤方面具有很大的潜力。该研究首次证明了两种阳离子分子可以基于单体和激基类似发射分别用于活细胞中核仁与溶酶体（1）/线粒体（2）的双色成像，更重要的是，可用于诊断细胞损伤。
