Pal Deepika, Kaur Navjot, Sudan Sarabjeet Kour, Bisht Bhawana, Krishnamurthi Srinivasan, Mayilraj Shanmugam
MTCC-Microbial Type Culture Collection and Gene Bank, CSIR- Institute of Microbial Technology, Chandigarh 160 036, India.
Division of protein science and Engineering, CSIR- Institute of Microbial Technology, Chandigarh 160 036, India.
Int J Syst Evol Microbiol. 2018 May;68(5):1719-1724. doi: 10.1099/ijsem.0.002736. Epub 2018 Apr 4.
A Gram-stain-negative, rod-shaped, aerobic, straw yellow, motile strain, designated KNDSW-TSA6, belonging to the genus Acidovorax, was isolated from a water sample of the river Ganges, downstream of the city of Kanpur, Uttar Pradesh, India. Cells were aerobic, non-endospore-forming and motile with single polar flagella. It differed from its phylogenetically related strains by phenotypic characteristics such as hydrolysis of urea, gelatin, casein and DNA, and the catalase reaction. The major fatty acids were C16 : 1ω7c/C16 : 1ω6c, C16 : 0 and C18 : 1ω7c/C18 : 1ω6c. Phylogenetic analysis based on 16S rRNA and housekeeping genes (gyrb, recA and rpoB gene sequences), confirmed its placement within the genus Acidovorax as a novel species. Strain KNDSW-TSA6 showed highest 16S rRNA sequence similarity to Acidovorax soli BL21 (98.9 %), Acidovorax delafieldii ATCC 17505 (98.8 %), Acidovorax temperans CCUG 11779 (98.2 %), Acidovorax caeni R-24608 (97.9 %) and Acidovorax radicis N35 (97.6 %). The digital DNA-DNA hybridization and average nucleotide identity values calculated from whole genome sequences between strain KNDSW-TSA6 and the two most closely related strains A. soli BL21 and A. delafieldii ATCC 17505 were below the threshold values of 70 and 95 % respectively. Thus, the data from the polyphasic taxonomic analysis clearly indicates that strain KNDSW-TSA6 represents a novel species, for which the name Acidovorax kalamii sp. nov. is proposed. The type strain is Acidovorax kalamii (=MTCC 12652=KCTC 52819=VTCC-B-910010).
从印度北方邦坎普尔市下游的恒河河水样本中分离出一株革兰氏阴性、杆状、需氧、稻草黄色、具运动性的菌株，命名为KNDSW - TSA6，属于嗜酸菌属。细胞为需氧型，不形成芽孢，具单根极生鞭毛，可运动。它在尿素、明胶、酪蛋白和DNA水解以及过氧化氢酶反应等表型特征方面与其系统发育相关菌株不同。主要脂肪酸为C16 : 1ω7c/C16 : 1ω6c、C16 : 0和C18 : 1ω7c/C18 : 1ω6c。基于16S rRNA和看家基因（gyrb、recA和rpoB基因序列）的系统发育分析证实，它在嗜酸菌属内属于一个新物种。菌株KNDSW - TSA6与嗜酸土壤杆菌BL21（98.9 %）、德拉菲尔德嗜酸菌ATCC 17505（98.8 %）、温和嗜酸菌CCUG 11779（98.2 %）、嗜铁嗜酸菌R - 24608（97.9 %）和嗜酸根瘤菌N35（97.6 %）的16S rRNA序列相似性最高。根据全基因组序列计算，菌株KNDSW - TSA6与最密切相关的两株菌株——嗜酸土壤杆菌BL21和德拉菲尔德嗜酸菌ATCC 17505之间的数字DNA - DNA杂交值和平均核苷酸同一性值分别低于70%和95%的阈值。因此，多相分类分析的数据清楚地表明，菌株KNDSW - TSA6代表一个新物种，为此提议将其命名为嗜酸卡氏菌（Acidovorax kalamii sp. nov.）。模式菌株为嗜酸卡氏菌（=MTCC 12652=KCTC 52819=VTCC - B - 910010）。
