Sudan Sarabjeet Kour, Pal Deepika, Bisht Bhawana, Kumar Narender, Chaudhry Vasvi, Patil Prabhu, Sahni Girish, Mayilraj Shanmugam, Krishnamurthi Srinivasan
Division of Protein Science and Engineering, CSIR-Institute of Microbial Technology, Chandigarh, 160 036, India.
MTCC-Microbial Type Culture Collection and Gene Bank, CSIR-Institute of Microbial Technology, Chandigarh, 160 036, India.
Int J Syst Evol Microbiol. 2018 Jan;68(1):402-408. doi: 10.1099/ijsem.0.002520. Epub 2017 Dec 14.
A bacterial strain, designated ASS-1, was isolated and identified from a sediment sample of the river Ganges, Allahabad, India. The strain was Gram-stain-negative, formed straw-yellow pigmented colonies, was strictly aerobic, motile with a single polar flagellum, and positive for oxidase and catalase. The major fatty acids were C16 : 1ω7c/ 16 : 1 C16 : 1ω6c, C18 : 1ω7c and C16 : 0. Sequence analysis based on the 16S rRNA gene revealed that strain ASS-1 showed high similarity to Pseudomonas guguanensis CC-G9A (98.2 %), Pseudomonas alcaligenes ATCC 14909 (98.2 %), Pseudomonas oleovorans DSM 1045 (98.1 %), Pseudomonas indolxydans IPL-1 (98.1 %) and Pseudomonas toyotomiensis HT-3 (98.0 %). Analysis of its rpoB and rpoD housekeeping genes confirmed its phylogenetic affiliation and showed identities lower than 93 % with respect to the closest relatives. Phylogenetic analysis based on the 16S rRNA, rpoB, rpoD genes and the whole genome assigned it to the genus Pseudomonas. The results of digital DNA-DNA hybridization based on the genome-to-genome distance calculator and average nucleotide identity revealed low genome relatedness to its close phylogenetic neighbours (below the recommended thresholds of 70 and 95 %, respectively, for species delineation). Strain ASS-1 also differed from the related strains by some phenotypic characteristics, i.e. growth at pH 5.0 and 42 °C, starch and casein hydrolysis, and citrate utilization. Therefore, based on data obtained from phenotypic and genotypic analysis, it is evident that strain ASS-1 should be regarded as a novel species within the genus Pseudomonas, for which the name Pseudomonasfluvialis sp. nov. is proposed. The type strain is ASS-1 (=KCTC 52437=CCM 8778).
从印度阿拉哈巴德恒河的沉积物样本中分离并鉴定出一株名为ASS-1的细菌菌株。该菌株革兰氏染色阴性，形成稻草黄色色素菌落，严格需氧，具单根极鞭毛可运动，氧化酶和过氧化氢酶呈阳性。主要脂肪酸为C16 : 1ω7c/ 16 : 1 C16 : 1ω6c、C18 : 1ω7c和C16 : 0。基于16S rRNA基因的序列分析表明，菌株ASS-1与古关假单胞菌CC-G9A（98.2 %）、产碱假单胞菌ATCC 14909（98.2 %）、食油假单胞菌DSM 1045（98.1 %）、吲哚氧化假单胞菌IPL-1（98.1 %）和丰臣假单胞菌HT-3（98.0 %）高度相似。对其rpoB和rpoD管家基因的分析证实了其系统发育归属，且与其最亲近的亲缘种的同一性低于93 %。基于16S rRNA、rpoB、rpoD基因和全基因组的系统发育分析将其归入假单胞菌属。基于基因组到基因组距离计算器和平均核苷酸同一性的数字DNA-DNA杂交结果显示，其与近缘系统发育邻种的基因组相关性较低（分别低于物种界定推荐阈值70和95 %）。菌株ASS-1在一些表型特征上也与相关菌株不同，即pH 5.0和42 °C下的生长、淀粉和酪蛋白水解以及柠檬酸盐利用。因此，根据表型和基因型分析获得的数据，很明显菌株ASS-1应被视为假单胞菌属内的一个新物种，为此提出名为河流假单胞菌（Pseudomonasfluvialis sp. nov.）。模式菌株为ASS-1（=KCTC 52437=CCM 8778）。
