College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
Nutraceutical Research Division, Tasly Holding Group Co., Ltd., Tianjin 300040, PR China.
Int J Syst Evol Microbiol. 2019 Dec;69(12):3903-3909. doi: 10.1099/ijsem.0.003700.
A Gram-reaction-negative, peach-brown-pigmented, slightly curved-rod-shaped, aerobic, non-motile bacterium, designated GSA243-2, was isolated from fresh water samples collected from the Chishui River flowing through Maotai, Guizhou, south-west PR China. Phenotypic, chemotaxonomic and genomic traits were investigated. Results of phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate belonged to the genus . The closest phylogenetic relative was ATCC BAA-1852 (98.35 %). The major fatty acids were C and Cω6 and/or Cω7. The major respiratory quinone was ubiquinone Q-8 and the major polar lipid was phosphatidylethanolamine. Genome sequencing revealed a genome size of 3.67 Mbp and a G+C content of 61.17 mol%. Pairwise-determined whole genome average nucleotide identity values and digital DNA-DNA hybridization values suggested that strain GSA243-2 represents a new species, for which we propose the name sp. nov. with the type strain GSA243-2 (=CGMCC 1.16288=KCTC 62564).
一株革兰氏反应阴性、桃棕色、略弯的杆状、需氧、非运动细菌，被命名为 GSA243-2，从中国西南部贵州省茅台市流经的赤水河中采集的淡水样本中分离得到。对其进行了表型、化学分类和基因组特征研究。基于 16S rRNA 基因序列的系统发育分析结果表明，该分离株属于 属。最接近的亲缘关系种是 ATCC BAA-1852（98.35%）。主要脂肪酸为 C 和 Cω6 和/或 Cω7。主要呼吸醌为泛醌 Q-8，主要极性脂为磷脂酰乙醇胺。基因组测序显示基因组大小为 3.67 Mbp，G+C 含量为 61.17 mol%。全基因组平均核苷酸同一性值和数字 DNA-DNA 杂交值的成对测定表明，菌株 GSA243-2 代表一个新种，我们建议使用 GSA243-2（=CGMCC 1.16288=KCTC 62564）作为其模式株，命名为 sp. nov.。
