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用等电聚焦抗原对小鼠进行约氏疟原虫血液期疟疾免疫及体内免疫与T细胞致敏的相关性

Immunization of mice against blood-stage Plasmodium yoelii malaria with isoelectrically focused antigens and correlation of immunity with T-cell priming in vivo.

作者信息

De Souza J B, Playfair J H

机构信息

Department of Immunology, Middlesex Hospital Medical School, London, United Kingdom.

出版信息

Infect Immun. 1988 Jan;56(1):88-91. doi: 10.1128/iai.56.1.88-91.1988.

DOI:10.1128/iai.56.1.88-91.1988
PMID:2961703
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC259239/
Abstract

Mice were immunized with lethal Plasmodium yoelii blood-stage malaria antigens that had been fractionated by isoelectric focusing using a variety of Ampholines over the range pH 3 to 10. Fractions were tested for their ability to protect against live challenge and to prime for parasite-specific T-cell help. Both activities exhibited three major peaks in the pH regions 4.5, 6.5, and 8, the pH 4.5 peak being the most consistently protective. There was a significant correlation between protection and T-helper-cell priming, particularly with antigens from the first peak, suggesting that T-cell priming represents an important component of the function of some protective malaria vaccines.

摘要

用经等电聚焦分离的致死性约氏疟原虫血液期疟疾抗原免疫小鼠，等电聚焦使用了一系列pH值范围为3至10的两性电解质。检测各组分抵抗活病原体攻击的能力以及启动寄生虫特异性T细胞辅助的能力。两种活性在pH值4.5、6.5和8区域均呈现三个主要峰，pH值4.5的峰具有最一致的保护性。保护性与T辅助细胞启动之间存在显著相关性，尤其是第一个峰的抗原，这表明T细胞启动是一些保护性疟疾疫苗功能的重要组成部分。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1611/259239/5b166536eea2/iai00073-0107-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1611/259239/5b166536eea2/iai00073-0107-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1611/259239/5b166536eea2/iai00073-0107-a.jpg

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Antibody recognition and isoelectrofocusing of antigens of the malaria parasite Plasmodium yoelii.约氏疟原虫疟原虫抗原的抗体识别及等电聚焦
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Vaccination of mice against malaria with soluble antigens. I. The effect of detergent, route of injection, and adjuvant.用可溶性抗原对小鼠进行疟疾疫苗接种。I. 去污剂、注射途径和佐剂的作用。
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Low parasite specific T cell response in clinically immune individuals with low grade Plasmodium falciparum parasitaemia.在患有低度恶性疟原虫血症的临床免疫个体中，寄生虫特异性T细胞反应较低。
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