Nishimura Wataru, Sakaue-Sawano Asako, Takahashi Satoru, Miyawaki Atsushi, Yasuda Kazuki, Noda Yasuko
a Department of Molecular Biology , International University of Health and Welfare School of Medicine , Narita , Chiba , Japan.
b Division of Anatomy, Bio-imaging and Neuro-cell Science, Jichi Medical University , Shimotsuke , Tochigi , Japan.
Islets. 2018 May 4;10(3):e1451282. doi: 10.1080/19382014.2018.1451282. Epub 2018 Apr 4.
Glucose metabolism is regulated by insulin, which is produced from β-cells in the pancreas. Because insulin is secreted into vessels in response to blood glucose, vascular structures of the pancreas, especially the relationship between vessels and β-cells, are important for physiological and pathological glucose metabolism. Here, we developed a system to visualize vessels surrounding mature β-cells expressing transcription factor MafA in a three-dimensional manner. Optical clearing of the pancreas prevented light scattering of fluorescence driven by the bacterial artificial chromosome (BAC)-mafA promoter in β-cells. Reconstruction of confocal images demonstrated mature β-cells and the glomerular-like structures of β-cell vasculatures labeled with DyLight 488-conjugated lectin in normal mice as well as in low-dose streptozotocin-injected diabetes model mice with reduced β-cell mass. This technological innovation of organ imaging can be used to investigate morphological changes in vascular structures during transplantation, regeneration and diabetes development.
葡萄糖代谢受胰岛素调节，胰岛素由胰腺中的β细胞产生。由于胰岛素会响应血糖分泌到血管中，胰腺的血管结构，尤其是血管与β细胞之间的关系，对生理和病理状态下的葡萄糖代谢至关重要。在此，我们开发了一种系统，能够以三维方式可视化围绕表达转录因子MafA的成熟β细胞的血管。胰腺的光学透明处理可防止β细胞中由细菌人工染色体（BAC）-mafA启动子驱动的荧光发生光散射。共聚焦图像重建显示，在正常小鼠以及低剂量链脲佐菌素注射诱导的β细胞数量减少的糖尿病模型小鼠中，成熟β细胞和用DyLight 488偶联凝集素标记的β细胞血管的肾小球样结构。这种器官成像技术创新可用于研究移植、再生和糖尿病发展过程中血管结构的形态变化。
