Department of Pharmaceutics and Pharmaceutical Technology, School of Pharmacy, The University of Jordan, Amman, 11942 Jordan.
Antimicrob Resist Infect Control. 2018 Apr 2;7:47. doi: 10.1186/s13756-018-0339-8. eCollection 2018.
Antibiotic resistance of environmental in hospitals could be increased due to extensive use of biocides resulting in serious infections. In this study, the prevalence of antibiotic resistance of environmental isolates of from hospitals and household settings were evaluated and compared. In addition, the association between biocide minimum inhibitory concentration (MIC) and multiple drug resistance (MDR) was investigated.
Environmental samples were collected from different homes and hospitals in Amman, Jordan. The isolates were identified phenotypically and by PCR. Antibiotic susceptibility tests and MIC of selected biocides were performed on the isolates. Screening for CTX-M group 1 was also performed.
Of 21 strains isolated, 47.6% were MDR and 67.9% were phenotypically identified as extended spectrum beta-lactamase (ESBL) producers. The occurrence of these ESBL isolates was comparable between household and hospital settings ( > 0.05). The MIC values of the biocides tested against all isolates were well below the in-use concentration of biocides. Moreover, the MICs of biocides were comparable between isolates from households and those from hospitals ( > 0.05). No association was found between MDR and biocide MIC (P > 0.05). Most of ESBL isolates harboured CTX-M 1.
The extensive use of biocides in hospitals is not associated with MDR nor does it affect the MIC of biocides against .
由于大量使用消毒剂，医院环境中的抗生素耐药性可能会增加，从而导致严重感染。在这项研究中，评估并比较了医院和家庭环境中分离的 环境分离株的抗生素耐药性流行率。此外，还研究了消毒剂最低抑菌浓度（MIC）与多重耐药（MDR）之间的关系。
从约旦安曼的不同家庭和医院采集环境样本。通过表型和 PCR 对分离株进行鉴定。对分离株进行抗生素敏感性试验和选定消毒剂的 MIC 测定。还对 CTX-M 组 1 进行了筛选。
在所分离的 21 株菌株中，47.6%为 MDR，67.9%表型鉴定为产超广谱β-内酰胺酶（ESBL）。家庭和医院环境中这些 ESBL 分离株的发生率相当（>0.05）。所有分离株对消毒剂的 MIC 值均远低于消毒剂的实际使用浓度。此外，家庭和医院分离株之间消毒剂 MIC 值相当（>0.05）。未发现 MDR 与消毒剂 MIC 值之间存在关联（P>0.05）。大多数 ESBL 分离株携带 CTX-M 1。
医院大量使用消毒剂与 MDR 无关，也不会影响消毒剂对 的 MIC 值。
