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传统草药三果木水提取物和甲醇提取物的植物化学分析及体外生化特性研究

Phytochemical analysis and In-vitro Biochemical Characterization of aqueous and methanolic extract of Triphala, a conventional herbal remedy.

作者信息

Parveen Romana, Shamsi Tooba Naz, Singh Gurjeet, Athar Teeba, Fatima Sadaf

机构信息

Department of Biotechnology, Jamia Millia Islamia, New Delhi 110025, India.

Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh 201303, India.

出版信息

Biotechnol Rep (Amst). 2018 Feb 8;17:126-136. doi: 10.1016/j.btre.2018.02.003. eCollection 2018 Mar.

Abstract

Triphala, an Indian ayurvedic triherbal formulation, is an equiproportional mixture of fruits of three herbs, (), () and (). The present study focused on phytocompounds detection and comparative analysis of various biochemical activities in the aqueous and methanolic extracts of triphala and its constituting herbs. Antioxidant activity was determined by 1, 1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), super oxide dismutase (SOD), catalase assay. Antibacterial potential was determined by broth dilution and agar well diffusion assays. Results revealed the presence of valuable bioactive compounds such as flavonoids, alkaloids, phenols, etc which might be responsible for biochemical activities. Extracts exhibited satisfactory radical-scavenging activity comparable with ascorbic acid. Methanolic extracts demonstrated higher antioxidant activity compared to aqueous extract. Extracts showed promising antibacterial potential against tested strain comparable to ampicillin. Hence, it can be concluded that triphala may be a promising candidate in pharmaceuticals and future medicine.

摘要

三果木,一种印度阿育吠陀三草药配方,是三种草药(诃子、毛诃子和余甘子)果实的等量混合物。本研究聚焦于三果木及其组成草药的水提取物和甲醇提取物中植物化合物的检测以及各种生化活性的比较分析。通过1,1-二苯基-2-苦基肼(DPPH)、铁还原抗氧化能力(FRAP)、超氧化物歧化酶(SOD)、过氧化氢酶测定来确定抗氧化活性。通过肉汤稀释法和琼脂孔扩散法来确定抗菌潜力。结果显示存在黄酮类、生物碱、酚类等有价值的生物活性化合物,这些可能是生化活性的原因。提取物表现出与抗坏血酸相当的令人满意的自由基清除活性。甲醇提取物相比于水提取物表现出更高的抗氧化活性。提取物对受试菌株显示出与氨苄青霉素相当的有前景的抗菌潜力。因此,可以得出结论,三果木可能是制药和未来医学中一个有前景的候选物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c0d/5881245/5e56804aaf58/gr1.jpg

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