Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, 225009, People's Republic of China.
College of Bioscience and Biotechnology, Yangzhou University, Yangzhou, 225009, People's Republic of China.
Arch Pharm Res. 2018 Jun;41(6):625-632. doi: 10.1007/s12272-018-1026-2. Epub 2018 Apr 4.
Three new neolignans, fordianoles A-C (1-3), characterized as (7S,8R)-4-hydroxy-3,3',5'-trimethoxy-8',9'-dinor-8,4'-oxyneolignan-7,7',9-triol, (7R,8R)-4-hydroxy-3,3',5'-trimethoxy-8',9'-dinor-8,4'-oxyneolignan-7,7',9-triol, and (7R,8R)-4-hydroxy-3,3',5'-trimethoxy-8,4'-oxyneolignan-7,9,9'-triol-7'-one, together with an unusual γ-lactone, 3-(3,4-dihydroxyphenyl)-4-pentanolide (4), and twenty-five known compounds (5-29) were isolated from the aerial parts of Viburnum fordiae Hance. Their structures including absolute configurations were determined by spectroscopic and chemical methods. Among them, compounds 6, 7, 11-15, 17-28 were isolated from the Viburnum genus for the first time. The anti-inflammatory and antioxidant activities of all compounds were evaluated in vitro. Compounds 15, 19, 20 and 29 showed significant inhibitory activity on NO production in RAW264.7 cells stimulated by LPS with IC values ranging from 8.60 to 13.92 μM. Meanwhile, compounds 1-4, 15, 19, 20, 22, 23, 25, 26 and 29 exhibited varying antioxidant activities through DPPH, ABTS free radical scavenging and FRAP assays.
从 Viburnum fordiae Hance 的地上部分分离得到三个新的木脂素，分别为 fordianoles A-C（1-3），其结构特征为（7S,8R）-4-羟基-3,3',5'-三甲氧基-8',9'-去甲-8,4'-氧新木脂素-7,7',9-三醇、（7R,8R）-4-羟基-3,3',5'-三甲氧基-8',9'-去甲-8,4'-氧新木脂素-7,7',9-三醇和（7R,8R）-4-羟基-3,3',5'-三甲氧基-8,4'-氧新木脂素-7,9,9'-三醇-7'-酮，此外还分离得到一个不常见的γ-内酯 3-(3,4-二羟基苯基）-4-戊醇酸内酯（4）和二十五个已知化合物（5-29）。通过光谱和化学方法确定了它们的结构，包括绝对构型。其中，化合物 6、7、11-15、17-28 为首次从 Viburnum 属中分离得到。所有化合物的抗炎和抗氧化活性均在体外进行了评估。化合物 15、19、20 和 29 对 LPS 刺激的 RAW264.7 细胞中 NO 生成的抑制活性最强，IC50 值范围为 8.60-13.92 μM。同时，化合物 1-4、15、19、20、22、23、25、26 和 29 通过 DPPH、ABTS 自由基清除和 FRAP 测定显示出不同的抗氧化活性。
