Department of Rheumatism and Immunology, Tai'an Central Hospital, Tai'an, Shandong 271000, P.R. China.
Department of Geriatric Diseases, Tai'an Central Hospital, Tai'an, Shandong 271000, P.R. China.
Mol Med Rep. 2018 Jun;17(6):7966-7972. doi: 10.3892/mmr.2018.8839. Epub 2018 Apr 3.
There are several reports in the literature regarding microRNA (miR)‑130b. It has been reported that miR‑130b is involved in several diseases. The present study aimed to understand the association between the levels of miR‑130b and lupus nephritis in patients. A total of 61 blood samples were collected and the expression level of miR‑130b was determined. The online miRNA database was then searched using the 'seed sequence' located within the 3'‑untranslated region of the target gene. Linear analysis and a luciferase assay were performed to understand the regulatory association between miR‑130b and phosphatase and tensin homolog (PTEN). In addition, reverse transcription‑polymerase chain reaction and western blot analyses were performed to examine the mRNA and protein expression levels of PTEN among individuals with lupus nephritis (n=28) and those without lupus nephritis (n=31), and in mesangial cells treated with scramble control, miR‑130b mimics, PTEN small interfering (si)RNA and miR‑130b inhibitors. In addition mesangial cells were treated with scramble control, miR‑130b mimics, PTEN siRNA and miR‑130b inhibitors to investigate the affect of miR‑130b and PTEN on the viability and apoptosis of mesangial cells. The results demonstrated that miR‑130b was downregulated in the hormone‑resistant group of lupus nephritis patients. PTEN was a virtual target of miR‑130b. There was a negative regulatory association between miR‑130b and PTEN. The mRNA and protein expression levels of PTEN were increased in the hormone‑resistant group. miR‑130b decreased the expression of PTEN. miR‑130b negatively interfered with the viability of mesangial cells and PTEN positively interfered with the viability of mesangial cells. miR‑130b accelerated apoptosis and PTEN inhibited apoptosis. Taken together, the results showed that miR‑130b was upregulated in the lupus nephritis group. PTEN was a virtual target of miR‑130b, and there was a negative regulatory association between miR‑130b and PTEN. miR‑130b and PTEN interfered with the viability and apoptosis of the mesangial cells.
有几项关于 microRNA (miR) -130b 的文献报道。据报道，miR-130b 参与了多种疾病。本研究旨在了解 miR-130b 水平与狼疮肾炎患者之间的关系。共采集 61 份血样，检测 miR-130b 的表达水平。然后使用位于靶基因 3'-UTR 内的“种子序列”在在线 miRNA 数据库中进行搜索。进行线性分析和荧光素酶测定，以了解 miR-130b 与磷酸酶和张力蛋白同源物（PTEN）之间的调控关联。此外，还进行了逆转录-聚合酶链反应和 Western blot 分析，以检查狼疮肾炎患者（n=28）和无狼疮肾炎患者（n=31）以及用 scramble 对照、miR-130b 模拟物、PTEN 小干扰 (si)RNA 和 miR-130b 抑制剂处理的系膜细胞中 PTEN 的 mRNA 和蛋白表达水平。此外，用 scramble 对照、miR-130b 模拟物、PTEN siRNA 和 miR-130b 抑制剂处理系膜细胞，以研究 miR-130b 和 PTEN 对系膜细胞活力和凋亡的影响。结果表明，miR-130b 在狼疮肾炎患者的激素耐药组中下调。PTEN 是 miR-130b 的虚拟靶标。miR-130b 与 PTEN 之间存在负调控关系。PTEN 的 mRNA 和蛋白表达水平在激素耐药组中增加。miR-130b 降低了 PTEN 的表达。miR-130b 负干扰系膜细胞的活力，PTEN 正干扰系膜细胞的活力。miR-130b 加速细胞凋亡，PTEN 抑制细胞凋亡。综上所述，结果表明 miR-130b 在狼疮肾炎组中上调。PTEN 是 miR-130b 的虚拟靶标，miR-130b 与 PTEN 之间存在负调控关系。miR-130b 和 PTEN 干扰系膜细胞的活力和凋亡。
