Department of Ophthalmology, The Second People's Hospital of Jinan, Jinan, Shandong 250001, P.R. China.
Department of Ophthalmology, The Second Hospital of Shandong University, Jinan, Shandong 250031, P.R. China.
Mol Med Rep. 2018 Jun;17(6):7774-7780. doi: 10.3892/mmr.2018.8841. Epub 2018 Apr 3.
Diabetic retinopathy (DR), one of the most common complications of late‑phase diabetes, is associated with the ectopic apoptosis of microvascular cells. Gastrodin, a phenolic glucoside derived from Gastrodia elata Blume, has been reported to have antioxidant and anti‑inflammation activities. The aim of the present study was to investigate the effects of gastrodin on high glucose (HG)‑induced human retinal endothelial cell (HREC) injury and its underlying mechanism. The results demonstrated that HG induced cell apoptosis in HRECs, which was accompanied by increased levels of reactive oxygen species production. Gastrodin treatment significantly alleviated HG‑induced apoptosis and oxidative stress. Furthermore, HG stimulation decreased the levels of SIRT1, which was accompanied by an increase in Toll‑like receptor 4 (TLR4) expression and the levels of phosphorylated nuclear factor (NF)‑κBp65. However, the administration of gastrodin significantly inhibited the activation of the sirtuin 1 (SIRT1)/TLR4/NF‑κBp65 signaling pathway in HRECs exposed to HG. Collectively, the present study demonstrated that gastrodin may be effective against HG‑induced apoptosis and its action may be exerted through the regulation of the SIRT1/TLR4/NF‑κBp65 signaling pathway.
糖尿病性视网膜病变（DR）是糖尿病晚期最常见的并发症之一，与微血管细胞的异位凋亡有关。天麻素是从天麻中提取的酚苷类物质，具有抗氧化和抗炎作用。本研究旨在探讨天麻素对高糖（HG）诱导的人视网膜内皮细胞（HREC）损伤的作用及其机制。结果表明，HG 诱导 HRECs 细胞凋亡，同时伴有活性氧（ROS）产生增加。天麻素处理可显著减轻 HG 诱导的细胞凋亡和氧化应激。此外，HG 刺激降低了 SIRT1 的水平，同时 TLR4 表达和磷酸化核因子（NF）-κBp65 水平增加。然而，天麻素的给药显著抑制了 HG 处理的 HRECs 中 SIRT1/TLR4/NF-κBp65 信号通路的激活。综上所述，本研究表明天麻素可能对 HG 诱导的细胞凋亡有效，其作用可能是通过调节 SIRT1/TLR4/NF-κBp65 信号通路发挥的。
