College of Biotechnology, Dalian Polytechnic University, Liaoning, 116000, P. R. China.
Department of Biotechnology, Hankyong National University, Anseong, 17579, Republic of Korea.
J Microbiol. 2018 May;56(5):331-336. doi: 10.1007/s12275-018-8098-z. Epub 2018 May 2.
A Gram-positive, strictly aerobic, nonmotile, yellowish, coccus-rod-shaped bacterium (designated Gsoil 653) isolated from ginseng cultivating soil was characterized using a polyphasic approach to clarify its taxonomic position. The strain Gsoil 653 exhibited optimal growth at pH 7.0 on R2A agar medium at 30°C. Phylogenetic analysis based on 16S rRNA gene sequence similarities, indicated that Gsoil 653 belongs to the genus Terrabacter of the family Humibacillus, and was closely related to Terrabacter tumescens DSM 20308 (98.9%), Terrabacter carboxydivorans PY2 (98.9%), Terrabacter terrigena ON10 (98.8%), Terrabacter terrae PPLB (98.6%), and Terrabacter lapilli LR-26 (98.6%). The DNA G + C content was 70.5 mol%. The major quinone was MK-8(H). The primary polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidyl-ethanolamine. The predominant fatty acids were iso-C, iso-C, iso-C, and anteiso-C, as in the case of genus Terrabacter, thereby supporting the categorization of strain Gsoil 653. However, the DNA-DNA relatedness between Gsoil 653 and closely related strains of Terrabacter species was low at less than 31%. Moreover, strain Gsoil 653 could be both genotypically and phenotypically distinguished from the recognized species of the genus Terrabacter. This isolate, therefore, represents a novel species, for which the name Terrabacter ginsengisoli sp. nov. is proposed with the type strain Gsoil 653 (= KACC 19444 = LMG 30325).
一株从人参种植土壤中分离得到的革兰氏阳性、严格需氧、非运动、浅黄色、球菌-杆菌状细菌（命名为 Gsoil 653），通过多相分类方法进行了特征描述，以阐明其分类地位。菌株 Gsoil 653 在 R2A 琼脂培养基上，最适生长 pH 值为 7.0，最适生长温度为 30°C。基于 16S rRNA 基因序列相似性的系统发育分析表明，Gsoil 653 属于 Humibacillus 科的 Terrabacter 属，与 Terrabacter tumescens DSM 20308（98.9%）、Terrabacter carboxydivorans PY2（98.9%）、Terrabacter terrigena ON10（98.8%）、Terrabacter terrae PPLB（98.6%）和 Terrabacter lapilli LR-26（98.6%）最为接近。DNA G+C 含量为 70.5mol%。主要醌为 MK-8(H)。主要极性脂为磷脂酰甘油、双磷脂酰甘油、磷脂酰乙醇胺。主要脂肪酸为 iso-C15:0、iso-C17:0 3-OH、iso-C16:0 和 anteiso-C15:0，与 Terrabacter 属的情况一致，因此支持将菌株 Gsoil 653 归类为该属。然而，Gsoil 653 与亲缘关系较近的 Terrabacter 种之间的 DNA-DNA 同源性低于 31%。此外，菌株 Gsoil 653 在基因型和表型上均能与已鉴定的 Terrabacter 属种区分开来。因此，该分离株代表了一个新的种，提议将其命名为 Terrabacter ginsengisoli sp. nov.，其模式菌株为 Gsoil 653（=KACC 19444=LMG 30325）。
