Badial Rodolfo Miglioli, Dias Marina Carrara, Stuqui Bruna, Melli Patrícia Pereira Dos Santos, Quintana Silvana Maria, Bonfim Caroline Measso do, Cordeiro José Antônio, Rabachini Tatiana, Calmon Marilia de Freitas, Provazzi Paola Jocelan Scarin, Rahal Paula
Department of Biology, São Paulo State University - UNESP, São José do Rio Preto/SP Department of Obstetrics and Gynecology, Hospital of Ribeirão Preto School of Medicine - HC-FMRP Department of Gynecology and Obstetrics, Medical School of Ribeirão Preto, University of São Paulo - USP Department of Epidemiology and Public Health, Faculty of Medicine of São José do Rio Preto - FAMERP, São José do Rio Preto/SP, Brazil Institute of Pharmacology, University of Bern, Bern, Switzerland.
Medicine (Baltimore). 2018 Apr;97(14):e9545. doi: 10.1097/MD.0000000000009545.
HPV have been identified as high-risk and low-risk, depending on their association with the development of cancer. HPV infections can be facilitated by co-infection with HIV. Here, we investigated HPV prevalence and genotypes and the risk factors affecting HPV/HIV co-infection. Forty HIV-positive patients had 80 cervical swab samples collected in 2 consecutive years. Polymerase chain reaction and DNA direct sequencing were used to perform HPV genotyping. Statistical analyses were performed regarding risk factors for HPV/HIV co-infection and the occurrence of cervical lesions. HPV DNA was detected in 59 samples (73.75%), and high-risk HPVs were predominant (59.3%). The most prevalent type was HPV56 (17%), followed by HPV16 (15.3%). Patient age did not affect the risk of cervical cancer (P = .84) or HPV prevalence in different years (P = .25/P = .63). CD4 count also did not affect the risk for cervical lesions in the tested samples (P = .15/P = .28). Although the HIV viral load was not correlated with an increase in cervical lesion detection in the first group of analyzed samples (P = .12), it did affect cervical cancer risk in the group of samples analyzed in the following year (P = .045). HIV-infected patients presented a high prevalence of HPV co-infection, and HPV16 and HPV56 were the most prevalent genotypes. Considering this, it is possible that immunodeficiency can contribute to increased susceptibility to HPV56 infection in HIV-infected patients. The association between HIV viral load and the lesions also confirmed the importance of monitoring HIV/HPV co-infected patients with high HIV viral loads.
根据与癌症发展的关联，人乳头瘤病毒（HPV）已被分为高危型和低危型。HIV合并感染可促使HPV感染。在此，我们调查了HPV的流行情况、基因型以及影响HPV/HIV合并感染的危险因素。40名HIV阳性患者在连续两年内采集了80份宫颈拭子样本。采用聚合酶链反应和DNA直接测序进行HPV基因分型。对HPV/HIV合并感染的危险因素以及宫颈病变的发生情况进行了统计分析。在59份样本（73.75%）中检测到HPV DNA，其中高危型HPV占主导（59.3%）。最常见的类型是HPV56（17%），其次是HPV16（15.3%）。患者年龄不影响宫颈癌风险（P = 0.84）或不同年份的HPV流行率（P = 0.25/P = 0.63）。CD4细胞计数也不影响检测样本中宫颈病变的风险（P = 0.15/P = 0.28）。虽然在第一组分析样本中，HIV病毒载量与宫颈病变检测增加无关（P = 0.12），但在次年分析的样本组中，它确实影响宫颈癌风险（P = 0.045）。HIV感染患者中HPV合并感染的发生率很高，HPV16和HPV56是最常见的基因型。考虑到这一点，免疫缺陷可能会导致HIV感染患者对HPV56感染的易感性增加。HIV病毒载量与病变之间的关联也证实了监测HIV病毒载量高的HIV/HPV合并感染患者的重要性。
