Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), Instituto Agroalimentario de Aragón-IA2 (CITA-Universidad de Zaragoza), Avda. Montañana 930, 50059 Zaragoza, Spain.
Food Chem. 2018 Aug 15;257:182-188. doi: 10.1016/j.foodchem.2018.02.139. Epub 2018 Feb 27.
An accurate, fast, economic and simple method to determine carotenoids, tocopherols, retinol and cholesterol in lyophilised samples of ovine milk, muscle and liver and raw samples of fat, which are difficult to lyophilise, is sought. Those analytes have been studied in animal tissues to trace forage feeding and unhealthy contents. The sample treatment consisted of mild overnight saponification, liquid-liquid extraction, evaporation with vacuum evaporator and redissolution. The quantification of the different analytes was performed by the use of ultra-high performance liquid chromatography with diode-array detector for carotenoids, retinol and cholesterol and fluorescence detector for tocopherols. The retention times of the analytes were short and the resolution between analytes was very high. The limits of detection and quantification were very low. This method is suitable for all the matrices and analytes and could be adapted to other animal species with minor changes.
我们正在寻找一种准确、快速、经济且简单的方法，以测定绵羊奶、肌肉和肝脏冻干样品以及难以冻干的脂肪原始样品中的类胡萝卜素、生育酚、视黄醇和胆固醇。这些分析物已在动物组织中进行了研究，以追踪饲料和不健康成分。样品处理包括温和的过夜皂化、液液萃取、真空蒸发器蒸发和重溶解。不同分析物的定量采用带有二极管阵列检测器的超高效液相色谱法进行，对于生育酚采用荧光检测器。分析物的保留时间短，分析物之间的分辨率非常高。检测限和定量限非常低。该方法适用于所有基质和分析物，并且可以通过微小的改动应用于其他动物物种。
