Dou Nianxu, Sun Rongbo, Su Chengcheng, Ma Yue, Zhang Xuewei, Wu Mengguo, Hou Juncai
College of Food Science, Northeast Agricultural University, Harbin 150030, China.
Foods. 2022 Jul 14;11(14):2088. doi: 10.3390/foods11142088.
In this experiment, the effect of replacing milk fat with soybean fat body (25%, 50%, 75%, 100%) on the quality, antioxidant capacity and in vitro digestive characteristics of yogurt was investigated while maintaining the total fat content of the yogurt unchanged. The results showed that increasing the substitution amount of soy fat body for milk fat had little effect on the pH and acidity of yogurt during the storage period, while the physicochemical properties, degree of protein gel network crosslinking, saturated fatty acid content, PV value and TBARS value of the yogurt significantly decreased (p < 0.05). Meanwhile, protein content, solids content, unsaturated fatty acid content, tocopherol content and water holding capacity significantly increased (p < 0.05). Flavor analysis revealed that yogurts with soybean oil bodies were significantly different when compared to those without soybean oil bodies (p < 0.05), and yogurt with 25% substitution had the highest sensory score. After in vitro digestion, the free fatty acid release, antioxidant capacity and protein digestibility of soybean oil body yogurt were significantly higher (p < 0.05). The SDS-PAGE results showed that the protein hydrolysis of the soybean oil body yogurt was faster. Therefore, the use of an appropriate amount of soybean oil bodies to replace milk fat is able to enhance the taste of yogurt and improve the quality of the yogurt.
在本实验中,研究了用大豆脂肪体(25%、50%、75%、100%)替代乳脂肪对酸奶品质、抗氧化能力和体外消化特性的影响,同时保持酸奶的总脂肪含量不变。结果表明,在储存期间,增加大豆脂肪体对乳脂肪的替代量对酸奶的pH值和酸度影响较小,而酸奶的理化性质、蛋白质凝胶网络交联程度、饱和脂肪酸含量、PV值和TBARS值显著降低(p<0.05)。同时,蛋白质含量、固形物含量、不饱和脂肪酸含量、生育酚含量和持水能力显著增加(p<0.05)。风味分析表明,含有大豆油体的酸奶与不含大豆油体的酸奶相比有显著差异(p<0.05),25%替代量的酸奶感官评分最高。体外消化后,大豆油体酸奶的游离脂肪酸释放量、抗氧化能力和蛋白质消化率显著更高(p<0.05)。SDS-PAGE结果表明,大豆油体酸奶的蛋白质水解更快。因此,使用适量的大豆油体替代乳脂肪能够增强酸奶的口感并改善酸奶品质。