Boccaletto Pietro, Siddique Mohammad Abdul Momin, Cosson Jacky
University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Centre of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic.
University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Centre of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic; Department of Oceanography, Noakhali Science and Technology University, Noakhali 3814, Bangladesh.
Anim Reprod Sci. 2018 May;192:18-27. doi: 10.1016/j.anireprosci.2018.03.033. Epub 2018 Mar 31.
Proteomics techniques, such as two-dimensional polyacrylamide gel electrophoresis, mass spectrometry, and differential gel electrophoresis, have been extensively used to describe the protein composition of male gametes in different animals, mainly mammals. They have also provided a deeper understanding of protein functions involved in sperm processes, as in processes that in humans lead to male infertility. However, few studies focus on fish sperm proteomics and even fewer have tried to explore the proteomic profile of Sturgeon spermatozoa. Sturgeon is an endangered, ancient group of fish species exploited mostly for caviar. In this fish group, a part of the process that leads to final functional maturation of spermatozoa so as to have the capability to activate eggs during the fertilization process. This process has a broad similarity to post-testicular maturation in mammals; where spermatozoa leaving the testes must be mixed with seminal fluid along the transit through the Wolffian ducts to modify its surface membrane protein composition, leading to axonemal and acrosomal competence. The aim of this study was to review the current literature on various proteomic techniques, their usefulness in separating, identifying and studying the proteome composition of the fish spermatozoon, as well as their potential applications in studying the post-testicular maturation process in Sturgeon. Such understanding could lead to development of more sophisticated aquaculture techniques, favorable for sturgeon reproduction.
蛋白质组学技术，如二维聚丙烯酰胺凝胶电泳、质谱分析和差异凝胶电泳，已被广泛用于描述不同动物（主要是哺乳动物）雄性配子的蛋白质组成。它们还使人们对精子过程中涉及的蛋白质功能有了更深入的了解，比如在人类中导致男性不育的过程。然而，很少有研究关注鱼类精子蛋白质组学，甚至更少有人尝试探索鲟鱼精子的蛋白质组概况。鲟鱼是一种濒危的古老鱼类，主要因其鱼子酱而被捕捞。在这类鱼中，精子最终功能成熟从而在受精过程中具备激活卵子能力的部分过程。这个过程与哺乳动物的睾丸后成熟有广泛的相似性；离开睾丸的精子在通过沃尔夫管的过程中必须与精液混合，以改变其表面膜蛋白组成，从而获得轴丝和顶体能力。本研究的目的是回顾当前关于各种蛋白质组学技术的文献，它们在分离、鉴定和研究鱼类精子蛋白质组组成方面的有用性，以及它们在研究鲟鱼睾丸后成熟过程中的潜在应用。这样的理解可能会促成更先进的水产养殖技术的发展，有利于鲟鱼繁殖。
