Li Ping, Du Hao, Qiao Xin Mei, Liu Zhi Gang, Zhou Qiong, Wei Wei Qi
Marine College, Shandong University (Weihai), Weihai, Shandong, 264209, China; Sino-Czech Joint Laboratory for Fish Conservation and Biotechnology, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China; University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic.
Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China; Marine College, Shandong University (Weihai), Weihai, Shandong, 264209, China.
Anim Reprod Sci. 2019 Feb;201:1-11. doi: 10.1016/j.anireprosci.2018.12.002. Epub 2018 Dec 6.
Knowledge of conditions affecting sperm quality is essential for efficient culture of fish for commercial purposes and conservation of species. Two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time of flight mass spectrometry were used to characterize the proteomic profile of Acipenser dabryanus spermatozoa relative to motility and fertilization capacity. There were differential amounts of protein in 313 spots in spermatozoa of males classified to have relatively greater or lesser spermatozoa quality. The functions of 43 of 50 selected proteins were identified. The proteins in 14 spots were involved in metabolism, and of these, proteins in 11 spots were highly abundant in spermatozoa of males categorized to have spermatozoa of greater quality, including pyruvate kinase, enolase B, phosphoglycerate kinase, lactate dehydrogenase, cytosolic malate dehydrogenase, brain creatine kinase b, Ckmb protein, and nucleoside diphosphate kinase. The proteins involved in mechanics of flagellum movement were identified, including the dynein intermediate chain, radial spoke head 1 homolog; ropporin-1-like, Bardet-Biedl syndrome 5, ADP-ribosylation factor-like protein 3, tektin-4, gamma-actin, and tubulin cytoskeleton proteins to be differentially abundant in spermatozoa that were classified relatively greater or lesser quality. Heat shock proteins, copper/zinc superoxide dismutase and peroxiredoxins, which are involved in stress response were of differential abundance in spermatozoa from males with spermatozoa in the two different classification groups. Proteins were also detected that are involved in protein folding and binding, or hydrolase activity. The results are valuable for the prediction of sperm quality and for reproduction management in A. dabryanus and other threatened species.
了解影响精子质量的因素对于商业养鱼和物种保护的高效养殖至关重要。二维凝胶电泳和基质辅助激光解吸/电离飞行时间质谱用于表征达氏鲟精子相对于活力和受精能力的蛋白质组图谱。在被分类为精子质量相对较高或较低的雄性精子中，313个斑点中的蛋白质含量存在差异。确定了50种选定蛋白质中43种的功能。14个斑点中的蛋白质参与代谢，其中11个斑点中的蛋白质在被分类为精子质量较高的雄性精子中高度丰富，包括丙酮酸激酶、烯醇化酶B、磷酸甘油酸激酶、乳酸脱氢酶、胞质苹果酸脱氢酶、脑肌酸激酶b、Ckmb蛋白和核苷二磷酸激酶。确定了参与鞭毛运动机制的蛋白质，包括动力蛋白中间链、径向辐条头部1同源物；类roporin-1、巴德-比德尔综合征5、ADP-核糖基化因子样蛋白3、微管蛋白4、γ-肌动蛋白和微管细胞骨架蛋白在被分类为精子质量相对较高或较低的精子中差异丰富。参与应激反应的热休克蛋白、铜/锌超氧化物歧化酶和过氧化物酶在两个不同分类组中精子质量的雄性精子中差异丰富。还检测到参与蛋白质折叠和结合或水解酶活性的蛋白质。这些结果对于预测达氏鲟和其他濒危物种的精子质量以及繁殖管理具有重要价值。
