Department of Clinical Laboratory, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China (mainland).
Med Sci Monit. 2018 Apr 6;24:2031-2037. doi: 10.12659/msm.906596.
BACKGROUND D-dimer tests have been widely used to rule-out deep venous thrombosis (DVT), but with low specificity. Circulating microRNAs (miRNAs) are novel promising biomarkers in diverse diseases. The purpose of our study was to identify the diagnostic abilities of circulating miRNA-320a/b and to assess their correlation with plasma D-dimer in DVT and post-thrombotic syndrome (PTS) patients. MATERIAL AND METHODS Plasma samples were taken from 30 DVT patients, 30 PTS patients, and 30 age- and sex-matched healthy volunteers. Quantitative real-time PCR (qPCR) assay and turbidimetric immunoassay were conducted to assess the concentrations of miRNA-320a/b and D-dimer in plasma. RESULTS Circulating miRNA-320a and miRNA-320b were significantly upregulated in DVT patients with fold changes of 1.58 and 1.79, respectively. The receiver operating characteristic (ROC) curve analysis showed area under the curve (AUC) values of 0.70 (95% CI: 0.56-0.83) for miRNA-320a and 0.79 (95% CI: 0.67-0.90) for miRNA-320b. Moreover, plasma levels of miRNA-320b were associated with D-dimer values (r=0.52, 95% CI: 0.19-0.74) in DVT. However, no significant changes in plasma miRNA-320a/b and D-dimer were detected in PTS patients. CONCLUSIONS Compared with controls, circulating miRNA-320a/b was differentially expressed in DVT. Simultaneous detection of miRNA-320a/b with D-dimer may improve diagnostic accuracy of DVT.
背景 D-二聚体检测已广泛用于排除深静脉血栓形成（DVT），但特异性较低。循环 microRNAs（miRNAs）是多种疾病中有前途的新型生物标志物。本研究的目的是确定循环 miRNA-320a/b 的诊断能力，并评估其与 DVT 和血栓后综合征（PTS）患者血浆 D-二聚体的相关性。
材料和方法 采集 30 例 DVT 患者、30 例 PTS 患者和 30 例年龄和性别匹配的健康志愿者的血浆样本。采用实时定量 PCR（qPCR）和比浊免疫测定法检测血浆中 miRNA-320a/b 和 D-二聚体的浓度。
结果 DVT 患者中循环 miRNA-320a 和 miRNA-320b 显著上调，倍数变化分别为 1.58 和 1.79。受试者工作特征（ROC）曲线分析显示，miRNA-320a 的 AUC 值为 0.70（95%CI：0.56-0.83），miRNA-320b 的 AUC 值为 0.79（95%CI：0.67-0.90）。此外，DVT 患者血浆中 miRNA-320b 水平与 D-二聚体值相关（r=0.52，95%CI：0.19-0.74）。然而，PTS 患者的血浆 miRNA-320a/b 和 D-二聚体无明显变化。
结论 与对照组相比，DVT 患者循环 miRNA-320a/b 表达差异。联合检测 miRNA-320a/b 和 D-二聚体可能提高 DVT 的诊断准确性。
