Biddle Chuck J, George-Gay Beverly, Prasanna Praveen, Hill Emily M, Davis Thomas C, Verhulst Brad
Department of Nurse Anesthesia, Virginia Commonwealth University, Richmond, VA, USA.
Department of Anesthesiology, Virginia Commonwealth University Medical Center, Richmond, VA, USA.
Can J Infect Dis Med Microbiol. 2018 Feb 4;2018:1905360. doi: 10.1155/2018/1905360. eCollection 2018.
Anesthesia machines are known reservoirs of bacterial species, potentially contributing to healthcare associated infections (HAIs). An inexpensive, disposable, nonpermeable, transparent anesthesia machine wrap (AMW) may reduce microbial contamination of the anesthesia machine. This study quantified the density and diversity of bacterial species found on anesthesia machines after terminal cleaning and between cases during actual anesthesia care to assess the impact of the AMW. We hypothesized reduced bioburden with the use of the AMW.
In a prospective, experimental research design, the AMW was used in 11 surgical cases (intervention group) and not used in 11 control surgical cases. Cases were consecutively assigned to general surgical operating rooms. Seven frequently touched and difficult to disinfect "hot spots" were cultured on each machine preceding and following each case. The density and diversity of cultured colony forming units (CFUs) between the covered and uncovered machines were compared using Wilcoxon signed-rank test and Student's -tests.
There was a statistically significant reduction in CFU density and diversity when the AMW was employed.
The protective effect of the AMW during regular anesthetic care provides a reliable and low-cost method to minimize the transmission of pathogens across patients and potentially reduces HAIs.
麻醉机是已知的细菌储存库,可能会导致医疗相关感染(HAIs)。一种廉价、一次性、不可渗透、透明的麻醉机包裹物(AMW)可能会减少麻醉机的微生物污染。本研究对麻醉机在终末清洁后以及实际麻醉护理过程中不同病例之间发现的细菌种类的密度和多样性进行了量化,以评估AMW的影响。我们假设使用AMW可降低生物负荷。
在一项前瞻性实验研究设计中,11例手术病例(干预组)使用了AMW,11例对照手术病例未使用。病例被连续分配到普通外科手术室。在每个病例前后,对每台机器上7个经常接触且难以消毒的“热点”进行培养。使用Wilcoxon符号秩检验和学生t检验比较覆盖和未覆盖机器之间培养的菌落形成单位(CFU)的密度和多样性。
使用AMW时,CFU密度和多样性有统计学意义的降低。
AMW在常规麻醉护理期间的保护作用提供了一种可靠且低成本的方法,可最大限度地减少病原体在患者之间的传播,并可能减少医疗相关感染。