State and Local Joint Engineering Laboratory for Novel Functional Polymeric Materials, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 199 Ren'ai Road, Suzhou 215123, PR China.
State and Local Joint Engineering Laboratory for Novel Functional Polymeric Materials, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 199 Ren'ai Road, Suzhou 215123, PR China.
Colloids Surf B Biointerfaces. 2018 Jul 1;167:28-35. doi: 10.1016/j.colsurfb.2018.03.047. Epub 2018 Mar 28.
Clot and thrombus formation on surfaces that come into contact with blood is still the most serious problem for blood contacting devices. Despite many years of continuous efforts in developing hemocompatible materials, it is still of great interest to develop multifunctional materials to enable vascular cell selectivity (to favor rapid endothelialization while inhibiting smooth muscle cell proliferation) and improve hemocompatibility. In addition, biomaterial-associated infections also cause the failure of biomedical implants and devices. However, it remains a challenging task to design materials that are multifunctional, since one of their functions will usually be compromised by the introduction of another function. In the present work, the gold substrate was first layer-by-layer (LbL) deposited with a multilayered polyelectrolyte film containing chitosan (positively charged) and a copolymer of sodium 4-vinylbenzenesulfonate (SS) and the "guest" adamantane monomer 1-adamantan-1-ylmethyl methacrylate (P(SS-co-Ada), negatively charged) via electro-static interactions, referred to as Au-LbL. The chitosan and P(SS-co-Ada) were intended to provide, respectively, resistance to bacteria and heparin-like properties. Then, "host" β-cyclodextrin derivatives bearing seven lysine ligands (CD-L) were immobilized on the Au-LbL surface by host-guest interactions between adamantane residues and CD-L, referred to as Au-LbL/CD-L. Finally, a versatile surface coating with fibrinolytic activity (lysis of nascent clots), vascular cell selectivity and antibacterial properties was developed.
在与血液接触的表面上形成血栓仍然是血液接触设备面临的最严重问题。尽管多年来一直在不断努力开发血液相容性材料，但开发多功能材料以实现血管细胞选择性（有利于快速内皮化，同时抑制平滑肌细胞增殖）和改善血液相容性仍然具有很大的吸引力。此外，生物材料相关的感染也会导致生物医学植入物和设备的失效。然而，设计多功能材料仍然是一项具有挑战性的任务，因为它们的一种功能通常会因引入另一种功能而受到影响。在本工作中，首先通过静电相互作用将金基底逐层（LbL）沉积具有壳聚糖（带正电荷）和 4-乙烯基苯磺酸钠（SS）与金刚烷单体 1-金刚烷-1-基甲基丙烯酸甲酯（P(SS-co-Ada)（带负电荷）的多层聚电解质膜，称为 Au-LbL。壳聚糖和 P(SS-co-Ada)旨在分别提供抗细菌和肝素样性质。然后，通过金刚烷残基与 CD-L 之间的主客体相互作用，将带有七个赖氨酸配体的“主”β-环糊精衍生物（CD-L）固定在 Au-LbL 表面上，称为 Au-LbL/CD-L。最后，开发了一种具有纤维蛋白溶解活性（新生血栓的溶解）、血管细胞选择性和抗菌性能的多功能表面涂层。
