Hu Danhe, Wu Chao, Wang Ruichen, Yao Xiaohui, Nie Kai, Lv Quan, Fu Shihong, Yin Qikai, Su Wenzhe, Li Fan, Xu Songtao, He Ying, Liang Guodong, Li Xiangdong, Wang Huanyu
Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.
State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Pathogens. 2023 Mar 21;12(3):490. doi: 10.3390/pathogens12030490.
The Tembusu virus (TMUV), a member of the Flaviviridae family, can be transmitted via mosquitoes and cause poultry disease. In 2020, a strain of TMUV (YN2020-20) was isolated from mosquito samples collected in Yunnan province, China. In vitro experiments showed that TMUV-YN2020-20 produced a significant cytopathic effect (CPE) in BHK, DF-1, and VERO cells, while the CPE in C6/36 cells was not significant. Phylogenetic analysis revealed that the strain belonged to Cluster 3.2 and was closely related to the Yunnan mosquito-derived isolates obtained in 2012 and the Shandong avian-derived isolate obtained in 2014. Notably, TMUV-YN2020-20 developed five novel mutations (E-V358I, NS1-Y/F/I113L, NS4A-T/A89V, NS4B-D/E/N/C22S, and NS5-E638G) at loci that were relatively conserved previously. The results of this study demonstrate the continuous circulation and unique evolution of TMUV in mosquitoes in Yunnan province and suggest that appropriate surveillance should be taken.
坦布苏病毒（TMUV）是黄病毒科的成员，可通过蚊子传播并引发家禽疾病。2020年，从中国云南省采集的蚊子样本中分离出一株TMUV（YN2020 - 20）。体外实验表明，TMUV - YN2020 - 20在BHK、DF - 1和VERO细胞中产生了显著的细胞病变效应（CPE），而在C6/36细胞中的CPE不显著。系统发育分析显示，该毒株属于3.2簇，与2012年获得的云南蚊子源分离株和2014年获得的山东禽类源分离株密切相关。值得注意的是，TMUV - YN2020 - 20在先前相对保守的位点发生了五个新的突变（E - V358I、NS1 - Y/F/I113L、NS4A - T/A89V、NS4B - D/E/N/C22S和NS5 - E638G）。本研究结果证明了TMUV在云南省蚊子中的持续传播和独特进化，并建议应采取适当的监测措施。
