长链非编码 RNA PlncRNA-1 通过上调 TGF-β1 和下调 PHGDH 抑制乳腺癌的生长。

LncRNA PlncRNA-1 overexpression inhibits the growth of breast cancer by upregulating TGF-β1 and downregulating PHGDH.

机构信息

Department of Pathology, The People's Hospital of Dongying, No. 317, South Road, East District, Dongying, 257000, Shandong, China.

Department of Oncology, The People's Hospital of Dongying, Dongying, 257000, Shandong, China.

出版信息

Breast Cancer. 2018 Sep;25(5):619-625. doi: 10.1007/s12282-018-0858-4. Epub 2018 Apr 6.

DOI:10.1007/s12282-018-0858-4

PMID:29626321

Abstract

OBJECTIVE

To investigate the role of lncRNA PlncRNA-1 in the pathogenesis of breast cancer.

METHODS

A total of 78 patients with breast cancer as well as 48 healthy females were included in this study. Expression in tumor tissues and adjacent healthy tissues of breast cancer patients, as well as in breast tissues and serum of both patients and healthy control was detected by qRT-PCR. Cell proliferation was detected by CCK-8 assay, and cell apoptosis was tested by MTT assay. PlncRNA-1 overexpression cell lines were constructed and the effects on TGF-β1 as well as phosphoglycerate dehydrogenase (PHGDH) were explored by western blot.

RESULTS

Expression levels of PlncRNA-1 were significantly lower in tumor tissues than those in adjacent healthy tissues. Significantly lower expression levels of PlncRNA-1 were also found in breast cancer patients than those in healthy controls in both breast tissue and serum. Upregulation of PlncRNA-1 promoted the expression of TGF-β1, but inhibited the expression of PHGDH. LncRNA PlncRNA-1 overexpression reduced the proliferation rate, but increased the apoptosis rate of breast cancer cells, while treatment with TGF-β inhibitor reduced those effects of PlncRNA-1 overexpression.

CONCLUSION

LncRNA PlncRNA-1 overexpression inhibits the growth of breast cancer by upregulating TGF-β1 and downregulating PHGDH.

摘要

目的

探讨 lncRNA PlncRNA-1 在乳腺癌发病机制中的作用。

方法

本研究纳入了 78 例乳腺癌患者和 48 例健康女性。通过 qRT-PCR 检测乳腺癌患者肿瘤组织和相邻健康组织、患者和健康对照的乳腺组织和血清中的表达。通过 CCK-8 检测细胞增殖，通过 MTT 检测细胞凋亡。构建 PlncRNA-1 过表达细胞系，通过 Western blot 探讨其对 TGF-β1 和磷酸甘油酸脱氢酶（PHGDH）的影响。

结果

PlncRNA-1 在肿瘤组织中的表达水平明显低于相邻的健康组织。乳腺癌患者的乳腺组织和血清中 PlncRNA-1 的表达水平也明显低于健康对照组。上调 PlncRNA-1 促进了 TGF-β1 的表达，但抑制了 PHGDH 的表达。LncRNA PlncRNA-1 的过表达降低了乳腺癌细胞的增殖率，但增加了细胞凋亡率，而 TGF-β 抑制剂的处理则降低了 PlncRNA-1 过表达的这些作用。