I类人类白细胞抗原在不同血小板密度队列中的表达差异：血小板年龄的潜在指标？

Variation of class I HLA antigen expression among platelet density cohorts: a possible index of platelet age?

作者信息

Pereira J, Cretney C, Aster R H

机构信息

Center of Southeastern Wisconsin, Milwaukee 53233.

出版信息

Blood. 1988 Feb;71(2):516-9.

PMID:2962651

Abstract

Platelet alloantigens and other surface markers were studied in platelet cohorts of different mean density, using monoclonal and polyclonal probes. High density (HD) platelets expressed 12% more P1A1 molecules (46,942) than low density (LD) platelets (41,892). However, LD platelets carried 42% more HLA-A2 molecules (6,267 +/- 184) than HD platelets (4,406 +/- 232) (P less than .01) and 55% more class I HLA antigens (17,034 +/- 2,062 v 11,007 +/- 2,190) (P = .05). The platelet subpopulations did not differ in their content of glycoprotein (GP)IIb/IIIa complex or Baka antigen. The difference in expression of class I HLA antigens on HD and LD platelets is consistent with two possibilities: either class I HLA molecules are acquired from plasma or they are released into plasma as platelets age in circulation. Accordingly, class I HLA molecules may provide a useful marker of platelet age.

摘要

使用单克隆和多克隆探针，对不同平均密度血小板群体中的血小板同种抗原和其他表面标志物进行了研究。高密度（HD）血小板表达的P1A1分子（46,942个）比低密度（LD）血小板（41,892个）多12%。然而，LD血小板携带的HLA - A2分子（6,267±184个）比HD血小板（4,406±232个）多42%（P<0.01），且I类HLA抗原多55%（17,034±2,062对11,007±2,190）（P = 0.05）。血小板亚群在糖蛋白（GP）IIb/IIIa复合物或巴卡抗原的含量上没有差异。HD和LD血小板上I类HLA抗原表达的差异符合两种可能性：要么I类HLA分子是从血浆中获得的，要么随着血小板在循环中老化它们被释放到血浆中。因此，I类HLA分子可能提供一个有用的血小板老化标志物。