Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Alzahra University, Tehran, Iran.
Endocrine Physiology Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Gene. 2018 Jul 1;662:97-102. doi: 10.1016/j.gene.2018.04.003. Epub 2018 Apr 5.
Apelin is an adipocyte-derived peptide that plays an important role in regulation of energy homoeostasis and decrease of insulin resistance. We investigated the effects of exercise training on adipose tissue apelin expression in streptozotocin-nicotinamide induced diabetic rats. In this experimental study, 38 male Wistar rats were assigned to 4 groups: sedentary non-diabetic, trained non-diabetic, sedentary diabetic and trained diabetic. Type 2 diabetes mellitus (T2DM) was induced by intraperitoneal injection of streptozotocin and nicotinamide in adult rats. The trained groups ran 8-week on treadmill progressively for 45 min at a speed of 24 m/min and a 5% grade. After the training period, an intraperitoneal glucose tolerance test was performed in addition to the measurement of blood lipids and plasma apelin levels. Apelin mRNA levels in visceral adipose tissue were also analyzed. Two-way repeated measures analysis of variance (ANOVA) and Pearson correlation coefficient was used for analyzing data. P < 0.05 was considered to be statistically significant. Results indicated a significant decrease of insulin resistance followed by a significant increase of plasma levels and mRNA expression of apelin in trained diabetic compared with sedentary diabetic rats. Furthermore, apelin levels did not correlate significantly with body weight and epididymal fat weight in trained diabetic rats. In conclusion, the findings revealed that chronic exercise training, independently of body weight and fat pad weight by decrease of insulin resistance can increase apelin levels in visceral adipose tissue, thereby attenuating the progression of diabetes and decreasing insulin resistance in T2DM rats.
Apelin 是一种脂肪细胞衍生的肽，在调节能量平衡和降低胰岛素抵抗方面发挥着重要作用。我们研究了运动训练对链脲佐菌素-烟酰胺诱导的糖尿病大鼠脂肪组织 apelin 表达的影响。在这项实验研究中，38 只雄性 Wistar 大鼠被分为 4 组：安静型非糖尿病组、训练型非糖尿病组、安静型糖尿病组和训练型糖尿病组。2 型糖尿病（T2DM）通过腹腔注射链脲佐菌素和烟酰胺在成年大鼠中诱导。训练组在跑步机上进行 8 周的渐进式跑步，速度为 24m/min，坡度为 5%。训练期结束后，进行了腹腔内葡萄糖耐量试验，同时测量了血脂和血浆 apelin 水平。还分析了内脏脂肪组织中 apelin mRNA 水平。采用双因素重复测量方差分析（ANOVA）和 Pearson 相关系数进行数据分析。P<0.05 被认为具有统计学意义。结果表明，与安静型糖尿病组相比，训练型糖尿病组的胰岛素抵抗显著降低，血浆水平和 apelin mRNA 表达显著升高。此外，在训练型糖尿病大鼠中，apelin 水平与体重和附睾脂肪重量无显著相关性。结论：研究结果表明，慢性运动训练可降低胰岛素抵抗，增加内脏脂肪组织中 apelin 水平，从而减缓 2 型糖尿病大鼠的糖尿病进展，降低胰岛素抵抗，而与体重和脂肪垫重量无关。
