Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, 50670-420, Recife, Pernambuco, Brazil.
Departamento de Biotecnologia, Centro de Biotecnologia, Universidade Federal da Paraíba, 58051-970, João Pessoa, Paraíba, Brazil.
Regul Toxicol Pharmacol. 2018 Jun;95:289-297. doi: 10.1016/j.yrtph.2018.04.004. Epub 2018 Apr 4.
People broadly use medicinal plants although their safety and efficacy are often neglected. Several Cactaceae plants are used for medicinal purposes, but Pilosocereus gounellei (xique-xique) has been little studied for their pharmacological properties. In this study, a saline extract from Pilosocereus gounellei stem was evaluated for acute toxicity, effects on motor function, and antinociceptive activity in mice. Thin-layer chromatography and high-performance liquid chromatography revealed the presence of flavonoids and reducing sugars. No death of mice was noted with oral treatments at 2000 and 5000 mg/kg. Body weight gain, food intake, and water consumption were not affected with treatment at 2000 mg/kg. The number of segmented leukocytes was higher in the treated mice than in control, and reduced serum levels of cholesterol and triglycerides were detected after both extract treatments. No histological alterations in the liver, heart, lung, kidney, and spleen were detected. Open field and rotarod tests showed that the extract did not affect motor function and exploratory activity. Antinociceptive activity was detected in acetic acid-induced writhing, tail-immersion, and formalin tests, indicating interference with both non-inflammatory and inflammatory pain. In conclusion, the P. gounellei extract has antinociceptive activity in mice without showing toxicity and altering motor coordination.
人们广泛使用药用植物，尽管它们的安全性和疗效常常被忽视。有几种仙人掌科植物被用于药用目的，但 Pilosocereus gounellei（希克-希克）的药理特性研究甚少。在这项研究中，评估了来自 Pilosocereus gounellei 茎的盐水提取物的急性毒性、对运动功能的影响和对小鼠的镇痛活性。薄层层析和高效液相色谱法显示存在黄酮类化合物和还原糖。经口给予 2000 和 5000mg/kg 提取物时，未观察到小鼠死亡。体重增加、食物摄入和水消耗不受 2000mg/kg 处理的影响。与对照组相比，治疗组的分段白细胞数量更高，并且在两种提取物处理后均检测到血清胆固醇和甘油三酯水平降低。肝、心、肺、肾和脾均未观察到组织学改变。旷场和旋转棒试验表明，提取物不影响运动功能和探索性活动。在乙酸诱导的扭体、尾浸入和福尔马林试验中检测到镇痛活性，表明其干扰了非炎症性和炎症性疼痛。综上所述，P. gounellei 提取物在小鼠中具有镇痛活性，没有表现出毒性，也没有改变运动协调能力。
