University of Aberdeen Rowett Institute, Foresterhill, Aberdeen, AB25 2ZD, UK.
University of Aberdeen Rowett Institute, Foresterhill, Aberdeen, AB25 2ZD, UK; Department of Obstetrics and Gynaecology, University Medical Centre Groningen, University of Groningen, Groningen, The Netherlands.
Appetite. 2018 Jul 1;126:137-146. doi: 10.1016/j.appet.2018.04.002. Epub 2018 Apr 6.
Many pre-clinical models of binge-like eating involve predictable, scheduled, access to a palatable diet high in fat (HF), where access may be preceded by anticipatory behaviour. Here, to introduce spontaneity into the binge-type consumption of palatable diets, mice were allowed 2 h access on a random day once per week and at a random time within an 8 h window either side of the transition from dark phase to light phase. Despite normal intake of a stock diet prior to unpredictable access to HF diet, mice immediately initiated a substantial eating episode when presented with HF diet. Following this consumption, compensatory hypophagia was observed relative to stock diet-fed controls, and cumulative energy intakes converged. There were no effects of HF diet on body weight or body composition over a 12-week period. Binge-like consumption was also observed on unpredictable access to the complete liquid diet, chocolate Ensure, but not with a 10% sucrose solution. Binge-like responses to unpredictable access to HF diet or Ensure were similar in male and female mice, although there were effects of sex on caloric consumption from stock diet in the compensatory period following palatable diet intake, with higher intakes in females. The timing of the 2h access period relative to light phase transition affected intake of palatable diets, but less robustly than the equivalent effect on stock diet intake during the same timed periods - the diurnal patterning of energy intake was diet sensitive. The large spontaneous binge-like consumption on unpredictable access to either solid or liquid palatable diets in mice of either sex offers the potential to combine these attributes with other manipulations where a developing obesity is part of the binge-like eating phenotype.
许多类似 binge 的进食行为的临床前模型都涉及可预测的、有规律的、可获得高脂肪（HF）美味饮食的机会，在此之前可能会有预期行为。在这里，为了在美味饮食的 binge 型消费中引入自发性，每周随机一天让老鼠在 8 小时窗口的暗期到亮期过渡的任意时间内有 2 小时的进食机会。尽管在不可预测地接触 HF 饮食之前，老鼠正常摄入了储备饮食，但当它们接触到 HF 饮食时，立即开始了大量的进食。在这种消费之后，与储备饮食喂养的对照组相比，观察到补偿性的摄食量减少，累积能量摄入量趋同。在 12 周的时间内，HF 饮食对体重或身体成分没有影响。在不可预测地接触完整液体饮食巧克力 Ensure 时也观察到类似 binge 的消费，但接触 10%蔗糖溶液时则没有。不可预测地接触 HF 饮食或 Ensure 时，雄性和雌性老鼠的 binge 样反应相似，尽管在摄入美味饮食后的补偿期，由于雌性老鼠的摄入量较高，性别对储备饮食的卡路里摄入量有影响。2 小时进食期相对于亮期过渡的时间会影响美味饮食的摄入量，但不如在相同时间内对储备饮食摄入量的影响那么大——能量摄入量的昼夜节律模式对饮食敏感。雄性和雌性老鼠在不可预测地接触固体或液体美味饮食时，会大量自发地 binge 样消费，这为将这些属性与其他操纵结合起来提供了潜力，其中发展中的肥胖是 binge 样进食表型的一部分。
