Young J W, Steinman R M
Laboratory of Cellular Physiology and Immunology, Rockefeller University, New York, NY 10021.
Cell Immunol. 1988 Jan;111(1):167-82. doi: 10.1016/0008-8749(88)90061-5.
Human blood dendritic cells can be enriched to 40-80% purity by a new technique that is simpler, provides greater yields than prior methods, and resolves other populations that are enriched in monocytes and B and T lymphocytes. The procedure involves separation over two Percoll gradients after 0 and 2 days of culture, followed by removal of contaminating monocytes by panning on plates coated with human Ig. The resultant dendritic cell-enriched fraction is 10 times or more potent than the monocyte-enriched populations in stimulating T-cell proliferative responses to alloantigens and to Con A. Small B lymphocytes are inactive in both systems. Dendritic cells do not initiate mitogenesis to anti-CD3 monoclonal antibodies, a response for which the monocyte appears to be the critical accessory cell.
通过一种新技术可将人血树突状细胞富集至纯度为40%-80%，该技术更简单，比先前方法产量更高，且能分离出富含单核细胞、B淋巴细胞和T淋巴细胞的其他细胞群体。该程序包括在培养0天和2天后通过两个Percoll梯度进行分离，然后通过在包被人Ig的平板上淘选去除污染的单核细胞。所得富含树突状细胞的部分在刺激T细胞对同种异体抗原和刀豆蛋白A的增殖反应方面比富含单核细胞的群体强10倍或更多。小B淋巴细胞在这两个系统中均无活性。树突状细胞不会引发针对抗CD3单克隆抗体的有丝分裂，而单核细胞似乎是该反应的关键辅助细胞。
