Metlay J P, Puré E, Steinman R M
Rockefeller University, New York, New York.
J Exp Med. 1989 Jan 1;169(1):239-54. doi: 10.1084/jem.169.1.239.
Highly enriched populations of B lymphoblasts have been isolated after culture with anti-Ig-Sepharose and compared with dendritic cells as stimulators of CD4+ T cells in the murine MLR. The two populations clearly differed in phenotype; anti-Ig blasts were FcR+, B220+, 33D1-, while dendritic cells were FcR-, B220-, 33D1+. However, as MLR stimulators, they shared many common features. Both cells (a) expressed comparable levels of class II MHC products; (b) independently stimulated the primary MLR and the production of several T derived lymphokines including IL-2 and IL-4; and (c) were comparable in stimulating freshly sensitized T cells. However, the relative potencies of dendritic cells and anti-Ig blasts as primary MLR stimulators varied in a strain-dependent fashion. Only anti-Ig blasts could stimulate across an Mls barrier, being at least 100 times more active in stimulating Mls-mismatched, MHC-matched T cells, relative to syngeneic T cells. In contrast, dendritic cells were 10-30 times more potent than anti-Ig blasts when stimulating across an MHC barrier and were likewise more effective in binding MHC-disparate T cells to form the clusters in which the MLR was generated. Dendritic cell-T cell clustering was resistant to anti-LFA-1 mAb, while B blast-T cell clustering was totally blocked. Thus, anti-Ig B lymphoblasts and dendritic cells, two cell types which differ markedly in phenotype, also differ in efficiency and mechanism for initiating responses in allogeneic T cells.
用抗Ig - 琼脂糖凝胶培养后，已分离出高度富集的B淋巴母细胞群体，并将其与树突状细胞进行比较，作为小鼠混合淋巴细胞反应（MLR）中CD4 + T细胞的刺激物。这两个群体在表型上明显不同；抗Ig母细胞为FcR +、B220 +、33D1 -，而树突状细胞为FcR -、B220 -、33D1 +。然而，作为MLR刺激物，它们有许多共同特征。两种细胞（a）表达相当水平的II类MHC产物；（b）独立刺激原发性MLR和几种T衍生淋巴因子（包括IL - 2和IL - 4）的产生；（c）在刺激新致敏的T细胞方面相当。然而，树突状细胞和抗Ig母细胞作为原发性MLR刺激物的相对效力因品系而异。只有抗Ig母细胞能够跨越Mls屏障进行刺激，相对于同基因T细胞，在刺激Mls不匹配、MHC匹配的T细胞方面活性至少高100倍。相比之下，当跨越MHC屏障进行刺激时，树突状细胞的效力比抗Ig母细胞高10 - 30倍，并且在结合MHC不同的T细胞以形成产生MLR的簇方面同样更有效。树突状细胞 - T细胞簇对抗LFA - 1单克隆抗体有抗性，而B母细胞 - T细胞簇则完全被阻断。因此，抗Ig B淋巴母细胞和树突状细胞这两种在表型上明显不同的细胞类型，在启动同种异体T细胞反应的效率和机制上也有所不同。
