Electroanalytical Chemistry Research Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran.
Electroanalytical Chemistry Research Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran.
Bioelectrochemistry. 2018 Aug;122:142-148. doi: 10.1016/j.bioelechem.2018.03.012. Epub 2018 Mar 28.
The detection and inhibition of DNA damage are of great importance in the prevention and treatment of diseases. Developing a simple and sensitive tool for this purpose would be a chance to monitor the DNA damage and could be helpful in introducing some drugs which can prevent this phenomenon. Here, we report a novel and sensitive electrochemical biosensor based on DNA/Au nanoparticles (AuNPs) modified screen printed gold electrode (DNA/AuNPs/SPGE) to investigate the DNA damage process and also to study the protective behavior of deferoxamine (DFO). The proposed biosensor was fabricated by electrodeposition of AuNPs onto SPGE, followed by chemical immobilisation of thiol-terminated DNA. Cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and scanning electron microscopy (SEM) have been used to characterise this biosensor. Hydroxyl radical (OH), which is generated during the Fenton reaction, is responsible for the induced damage to DNA. EIS technique was applied to monitor the DNA damage, and the increase in charge transfer resistance (R) following the DNA damage, was considered as an indicator. Furthermore, the ability of the electrochemical screening system was proved by the investigation of the antioxidant effect of DFO in prohibiting the DNA damage.
检测和抑制 DNA 损伤对于疾病的预防和治疗非常重要。为此开发一种简单而灵敏的工具将是一个监测 DNA 损伤的机会，并有助于引入一些可以预防这种现象的药物。在这里，我们报告了一种基于 DNA/Au 纳米粒子（AuNPs）修饰的丝网印刷金电极（DNA/AuNPs/SPGE）的新型灵敏电化学生物传感器，用于研究 DNA 损伤过程，并研究去铁胺（DFO）的保护行为。该生物传感器通过 AuNPs 在 SPGE 上电沉积，然后进行巯基化 DNA 的化学固定化来制备。循环伏安法（CV）、电化学阻抗谱（EIS）和扫描电子显微镜（SEM）用于表征该生物传感器。在芬顿反应期间产生的羟基自由基（OH）是导致 DNA 损伤的原因。EIS 技术被用于监测 DNA 损伤，并且 DNA 损伤后电荷转移电阻（R）的增加被认为是一个指标。此外，通过研究 DFO 的抗氧化作用在抑制 DNA 损伤方面的能力，证明了电化学筛选系统的能力。
