用于基于阻抗法检测微小RNA的纸质电极组件
Paper-based electrode assemble for impedimetric detection of miRNA.
作者信息
Eksin Ece, Torul Hilal, Yarali Ece, Tamer Ugur, Papakonstantinou Pagona, Erdem Arzum
机构信息
Department of Analytical Chemistry, Faculty of Pharmacy, Ege University, Bornova, 35100, İzmir, Turkey.
Department of Analytical Chemistry, Faculty of Pharmacy, Gazi University, Etiler, 06330, Ankara, Turkey.
出版信息
Talanta. 2021 Apr 1;225:122043. doi: 10.1016/j.talanta.2020.122043. Epub 2020 Dec 30.
In the present work, a paper-based electrode assemble was developed and implemented to detect target microRNA 155 (miRNA 155) via electrochemical impedance spectroscopy (EIS) measurements. In this concept, gold nanoparticles (AuNPs) modified paper based electrode assemble system (AuNP-PE) was designed, and characterized by scanning electron microscopy (SEM), cyclic voltammetry (CV) and EIS measurements. The impedimetric detection of miRNA 155 was performed by measuring the fractional change at the charge transfer resistance (R). The detection limits were found as 33.8 nM in PBS and 93.4 nM in fetal bovine serum (FBS) medium, respectively. The selectivity of the proposed assay was tested against to non-complementary (NC) and mismatch (MM) miRNA sequences in the presence of mixture sample containing miRNA:NC (1:1) and miRNA:MM (1:1) in PBS (pH 7.40) or FBS. The analytical performance and the selectivity of impedimetric biosensor were also tested in FBS.
在本研究中，开发并实施了一种基于纸的电极组件，通过电化学阻抗谱（EIS）测量来检测目标微小RNA 155（miRNA 155）。在此概念中，设计了金纳米颗粒（AuNPs）修饰的基于纸的电极组件系统（AuNP-PE），并通过扫描电子显微镜（SEM）、循环伏安法（CV）和EIS测量对其进行了表征。通过测量电荷转移电阻（R）处的分数变化来进行miRNA 155的阻抗检测。在磷酸盐缓冲盐水（PBS）和胎牛血清（FBS）培养基中的检测限分别为33.8 nM和93.4 nM。在含有miRNA:非互补（NC）（1:1）和miRNA:错配（MM）（1:1）的混合样品存在下，于PBS（pH 7.40）或FBS中，针对非互补（NC）和错配（MM）miRNA序列测试了所提出测定法的选择性。还在FBS中测试了阻抗生物传感器的分析性能和选择性。
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