Gómez-López Modesto, Miliar-García Ángel, Pérez-Vielma Nadia Mabel, Lara-Padilla Eleazar, González-Díaz César Antonio
Escuela Superior de Medicina, Instituto Politécnico Nacional. Plan de San Luis y Díaz Mirón. C.P. 11360, CDMX, México.
Centro Interdisciplinario de Ciencias de la Salud-Unidad Santo Tomás, Instituto Politécnico Nacional. Av. Maestros C.P. 11340, CDMX, México.
J Electr Bioimpedance. 2020 Jun 25;11(1):31-37. doi: 10.2478/joeb-2020-0006. eCollection 2020 Jan.
The development of biosensors to identify molecular markers or specific genes is fundamental for the implementation of new techniques that allow the detection of specific Deoxyribonucleic acid (DNA) sequences in a fast, economic and simple way. Different detection techniques have been proposed in the development of biosensors. Electrical Bioimpedance Spectroscopy (EBiS) has been used for diagnosis and monitoring of human pathologies, and is recognized as a safe, fast, reusable, easy and inexpensive technique. This study proves the development of a complementary DNA (cDNA) biosensor based on measurements of EBiS and DNA's immobilization with no chemical modifications. The evaluation of its potential utility in the detection of the gene expression of three inflammation characteristic biomarkers (NLRP3, IL-1β and Caspase 1) is presented. The obtained results demonstrate that EBiS can be used to identify different gene expression patterns, measurements that were validated by Quantitative Polymerase Chain Reaction (qPCR). These results indicate the technical feasibility for a biosensor of specific genes through bioimpedance measurements on the immobilization of cDNA.
开发用于识别分子标记或特定基因的生物传感器，对于实现能够快速、经济且简便地检测特定脱氧核糖核酸（DNA）序列的新技术至关重要。在生物传感器的开发过程中，人们提出了不同的检测技术。生物电阻抗谱（EBiS）已被用于人类疾病的诊断和监测，并且被认为是一种安全、快速、可重复使用、简便且廉价的技术。本研究证明了基于EBiS测量和无需化学修饰的DNA固定化技术开发互补DNA（cDNA）生物传感器的可行性。文中展示了对其在检测三种炎症特征生物标志物（NLRP3、IL-1β和Caspase 1）基因表达方面潜在效用的评估。所得结果表明，EBiS可用于识别不同的基因表达模式，这些测量结果通过定量聚合酶链反应（qPCR）得到了验证。这些结果表明，通过对固定化cDNA进行生物阻抗测量来开发特定基因生物传感器具有技术可行性。
