Department of Tissue Engineering, Najafabad branch, Islamic Azad University, Najafabad, Iran.
Advanced Materials Research Center, Department of Materials Engineering, Najafabad Branch, Islamic Azad University, Najafabad, Iran.
J Mech Behav Biomed Mater. 2018 Jun;82:257-267. doi: 10.1016/j.jmbbm.2018.03.016. Epub 2018 Mar 15.
In this study, a bone cement consisting of poly methyl methacrylate (PMMA)-poly caprolactone (PCL)-fluorapatite (FA)-graphene oxide (GO) was synthesized as bone filler for application in orthopedic surgeries. The FA and GO particulates were homogenously distributed in the PMMA-PCL polymer matrix and no defects and agglomeration were found in the PMMA-PCL/FA/GO bone cement. The in-vitro bioactivity result exhibited that addition of FA and GO to the polymer cement (PMMA-PCL) improved the apatite formation ability on the surface of polymer. The results also showed that addition of FA to the polymer bone cement escalated the compressive strength and elastic modulus while reducing elongation to 8 ± 2%. However, after addition of GO into the PMMA-PCL/FA bone cement, both compressive strength and elongation considerably increased to 101 ± 5 MPa and 35 ± 6%, respectively. Furthermore, tensile tests exhibited that inclusion of GO was favorable in improving the tensile modulus, UTS and elongation of the PMMA-PCL/FA bone cement. The cytotoxicity test pointed out that MG63 osteoblast cells viability increased to 279 ± 15% after addition of FA and GO to the PMMA-PCL polymer bone cement. The DAPI (4',6-diamidino-2-phenylindole) staining demonstrated better spreading and attachment of MG63 cells on PMMA-PCL/FA/GO surface compared to the PMMA-PCL bone cements. These results confirm the suitable mechanical properties and favorable bioactivity along with high cells viability of PMMA-PCL/FA/GO bone cement, indicating its potentials for orthopedic applications.
在这项研究中，合成了一种由聚甲基丙烯酸甲酯（PMMA）-聚己内酯（PCL）-氟磷灰石（FA）-氧化石墨烯（GO）组成的骨水泥，用作骨科手术中的骨填充剂。FA 和 GO 颗粒均匀分布在 PMMA-PCL 聚合物基质中，在 PMMA-PCL/FA/GO 骨水泥中没有发现缺陷和团聚。体外生物活性结果表明，在聚合物水泥（PMMA-PCL）中添加 FA 和 GO 提高了聚合物表面的磷灰石形成能力。结果还表明，在聚合物骨水泥中添加 FA 可提高抗压强度和弹性模量，同时将伸长率降低至 8 ± 2%。然而，在 PMMA-PCL/FA 骨水泥中添加 GO 后，抗压强度和伸长率分别显著提高到 101 ± 5 MPa 和 35 ± 6%。此外，拉伸试验表明，GO 的加入有利于提高 PMMA-PCL/FA 骨水泥的拉伸模量、UTS 和伸长率。细胞毒性试验表明，在 PMMA-PCL 聚合物骨水泥中添加 FA 和 GO 后，MG63 成骨细胞的存活率增加到 279 ± 15%。DAPI（4',6-二脒基-2-苯基吲哚）染色表明，与 PMMA-PCL 骨水泥相比，MG63 细胞在 PMMA-PCL/FA/GO 表面具有更好的扩散和附着。这些结果证实了 PMMA-PCL/FA/GO 骨水泥具有合适的机械性能、良好的生物活性和高细胞活力，表明其在骨科应用中的潜力。
