Da-Costa Tânia Maris Pedrini Soares, Hidalgo Mirian Marubayashi, Consolaro Alberto, Lima Carlos Eduardo de Oliveira, Tanaka Evelise Ono, Itano Eiko Nakagawa
Department of Pathological Sciences, State University of Londrina, Londrina, Brazil.
Department of Dentistry, State University of Maringá, Maringá, Brazil.
Int Orthod. 2018 Jun;16(2):258-267. doi: 10.1016/j.ortho.2018.03.015. Epub 2018 Apr 6.
External apical root resorption as a consequence of orthodontic treatment is an inflammatory pathological process that results in permanent loss of tooth structure from the root apex.
This study aimed to investigate the diagnostic potential of human dentine fractions and salivary IgG in external apical root resorption.
Saliva samples were collected from 10 patients before (T0) and after 3 (T3), 6 (T6) and 12 (T12) months of orthodontic treatment. The total dentinal extract, obtained from human third molars, was fractioned by gel filtration chromatography in three fractions denominated FI, FII and FIII. The root resorption analysis of the upper central incisors was performed by digital image subtraction method. Reactivity of salivary IgG to antigenic fractions of dentine was determined by enzyme-linked immunosorbent assay (Elisa).
Regardless of treatment, FI dentin fraction with high MM (<300kDa) was the one that presented highest reactivity with salivary IgG. However, it was found higher salivary IgG reactivity for FII (69 to 45 kilodalton [kDa]) as compared to FIII (<45kDa) at (T6) and (T12), (P<0.05), the same periods in that the root resorptions were detected.
Our results suggest that FII human dentine fraction and salivary IgG have potential to be used in diagnosis and monitoring of external apical root resorption. The development of a practical and accessible biochemical test using saliva and FII dentine fraction may help in the prevention of severe root resorption.
正畸治疗导致的根尖外吸收是一种炎症性病理过程，会导致牙根尖的牙体结构永久性丧失。
本研究旨在探讨人牙本质组分和唾液IgG在根尖外吸收中的诊断潜力。
收集10例患者正畸治疗前（T0）、治疗3个月（T3）、6个月（T6）和12个月（T12）后的唾液样本。从人类第三磨牙获得的总牙本质提取物通过凝胶过滤色谱法分为三个组分，分别命名为FI、FII和FIII。采用数字图像减法对上前牙的牙根吸收情况进行分析。采用酶联免疫吸附测定（ELISA）法测定唾液IgG与牙本质抗原组分的反应性。
无论治疗情况如何，高分子量（<300kDa）的FI牙本质组分与唾液IgG的反应性最高。然而，在（T6）和（T12）时，发现FII（69至45千道尔顿[kDa]）的唾液IgG反应性高于FIII（<45kDa）（P<0.05），这两个时期均检测到牙根吸收。
我们的结果表明，FII人牙本质组分和唾液IgG有潜力用于根尖外吸收的诊断和监测。开发一种使用唾液和FII牙本质组分的实用且易于操作的生化检测方法可能有助于预防严重的牙根吸收。
