Deptment of Oral and Maxillofacial Surgery, State Key Laboratory of Oral Disease, West China School of Stomatology, Sichuan University, PR China.
State Key Laboratory of Oral Disease, West China School of Stomatology, Sichuan University, PR China.
Clin Chim Acta. 2018 Mar;478:188-193. doi: 10.1016/j.cca.2017.12.046. Epub 2017 Dec 29.
Orthodontically induced external apical root resorption (OIEARR) is one of the most severe complications of orthodontic treatment, which is hard to diagnose at early stage by merely radiographic examination. This study aimed to identify salivary metabolic products using unbiased metabolic profiling in order to discover biomarkers that may indicate OIEARR.
Unstimulated saliva samples were analyzed from 19 healthy orthodontic patients with EARR (n=8) and non-EARR (n=11). Metabolite profiling was performed using H Nuclear Magnetic Resonance (NMR) spectroscopy.
A total of 187 metabolites were found in saliva samples. With supervised partial least squares discriminant analysis and regression analysis, samples from 2 groups were well separated, attributed by a series of metabolites of interest, including butyrate, propane-1,2-diol, α-linolenic acid (Ala), α-glucose, urea, fumarate, formate, guanosine, purine, etc. Indicating the increased inflammatory responses in the periodontal tissues possibly associated with energy metabolism and oxidative stress.
The effective separation capacity of H NMR based metabolomics suggested potential feasibility of clinical application in monitoring periodontal and apical condition in orthodontic patients during treatment and make early diagnosis of OIEARR. Metabolites detected in this study need further validation to identify exact biomarkers of OIEARR. Saliva biomarkers may assist in diagnosis and monitoring of this disease.
正畸性牙外吸收（OIEARR）是正畸治疗中最严重的并发症之一，仅通过影像学检查很难在早期诊断。本研究旨在使用无偏代谢组学鉴定唾液代谢产物，以发现可能提示 OIEARR 的生物标志物。
从 19 名接受 EARR（n=8）和非 EARR（n=11）正畸治疗的健康正畸患者中采集非刺激性唾液样本。使用 H 核磁共振（NMR）光谱法进行代谢产物分析。
在唾液样本中发现了 187 种代谢物。通过有监督的偏最小二乘判别分析和回归分析，两组样本得到了很好的分离，这归因于一系列感兴趣的代谢物，包括丁酸盐、丙烷-1,2-二醇、α-亚麻酸（Ala）、α-葡萄糖、尿素、富马酸、甲酸盐、鸟苷、嘌呤等。这表明牙周组织的炎症反应增强，可能与能量代谢和氧化应激有关。
基于 H NMR 的代谢组学具有有效的分离能力，表明其在监测正畸治疗期间牙周和根尖状况方面具有潜在的临床应用可行性，可实现对 OIEARR 的早期诊断。本研究中检测到的代谢物需要进一步验证，以确定 OIEARR 的确切生物标志物。唾液生物标志物可能有助于该疾病的诊断和监测。
