McKenzie M A, Fawell S E, Cha M, Lenard J
Department of Physiology and Biophysics, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway 08854-5635.
Endocrinology. 1988 Feb;122(2):511-7. doi: 10.1210/endo-122-2-511.
Addition of mammalian insulin to a nutritionally rich, chemically defined culture medium affects Neurospora crassa "slime" (wall-less) cells, as indicated by enhancement of growth, extension of viability at the stationary phase of growth, alteration of morphology, and stimulation of glucose oxidation. Bovine, porcine, and recombinant human insulin had similar effects on growth and morphology, while proinsulin, reduced insulin, and several other proteins were inactive. Insulin added in the presence of excess antiinsulin antibody was without activity. Intact cells possessed high affinity insulin-binding sites, represented by a curvilinear Scatchard plot, suggesting that effects are mediated through insulin receptors on the cell surface. These findings establish a role for insulin or insulin-like molecules in regulating growth and metabolism in this fungal cell and demonstrate a close similarity to insulin effects on certain mammalian cells.
向营养丰富、化学成分明确的培养基中添加哺乳动物胰岛素会影响粗糙脉孢菌的“黏液”（无壁）细胞，表现为生长增强、生长稳定期活力延长、形态改变以及葡萄糖氧化受到刺激。牛胰岛素、猪胰岛素和重组人胰岛素对生长和形态有类似影响，而胰岛素原、还原胰岛素和其他几种蛋白质则无活性。在过量抗胰岛素抗体存在的情况下添加胰岛素没有活性。完整细胞具有高亲和力胰岛素结合位点，由曲线型Scatchard图表示，这表明这些作用是通过细胞表面的胰岛素受体介导的。这些发现确立了胰岛素或胰岛素样分子在调节这种真菌细胞生长和代谢中的作用，并证明了与胰岛素对某些哺乳动物细胞的作用极为相似。
