Morgan D L, Furlow T L, Menzel D B
Department of Pharmacology, Duke University Medical Center, Durham, North Carolina 27710.
Environ Res. 1988 Feb;45(1):108-17. doi: 10.1016/s0013-9351(88)80013-6.
In vivo O3 exposure followed by in vitro incubation was observed to cause inhibition of mouse RBC deformability. The requirement for in vitro incubation to allow expression of these effects and the potential role for oxidizable membrane components were investigated. Membrane sulfhydryls (SHs) and membrane ATPase are both susceptible to oxidation by O3 and are essential for maintaining RBC membrane deformability. RBC SH levels and ATPase activity were unchanged immediately after exposure of mice to filtered air (controls) or 1 ppm O3. After a subsequent 6-hr in vitro incubation, RBCs from control mice exhibited significant increases in membrane SH and ATPase activity, while SH levels and ATPase activity in RBCs from O3-exposed mice remained unchanged. Although the stimulus for increasing membrane SH and ATPase activity is unclear, these changes appear to be essential to maintaining RBC deformability in vitro and are inhibited by in vivo O3 exposure.
观察到体内臭氧暴露后进行体外孵育会导致小鼠红细胞变形性受到抑制。研究了体外孵育对这些效应表达的必要性以及可氧化膜成分的潜在作用。膜巯基（SHs）和膜ATP酶都易被臭氧氧化，并且对于维持红细胞膜变形性至关重要。将小鼠暴露于过滤空气（对照组）或1 ppm臭氧后，红细胞的SH水平和ATP酶活性立即没有变化。在随后6小时的体外孵育后，来自对照小鼠的红细胞膜SH和ATP酶活性显著增加，而来自臭氧暴露小鼠的红细胞中的SH水平和ATP酶活性保持不变。尽管增加膜SH和ATP酶活性的刺激因素尚不清楚，但这些变化似乎对于在体外维持红细胞变形性至关重要，并且会被体内臭氧暴露所抑制。
