Sayers Jessica, Payne Richard J, Winssinger Nicolas
School of Chemistry , The University of Sydney , Sydney , NSW 2006 , Australia . Email:
Department of Organic Chemistry , Faculty of Science , NCCR Chemical Biology , University of Geneva , Quai Ernest Ansermet 30 , 1211 Geneva , Switzerland . Email:
Chem Sci. 2017 Nov 22;9(4):896-903. doi: 10.1039/c7sc02736b. eCollection 2018 Jan 28.
The development of a rapid and chemoselective selenocystine-selenoester peptide ligation that operates at nanomolar reactant concentrations has been developed by utilising PNA templation. Kinetic analysis of the templated peptide ligation revealed that the selenocystine-selenoester reaction was 10 times faster than traditional native chemical ligation at cysteine and to our knowledge is the fastest templated ligation reaction reported to date. The efficiency and operational simplicity of this technology is highlighted through the formation of hairpin molecular architectures and in a novel paper-based lateral flow assay for the rapid and sequence specific detection of oligonucleotides, including miRNA in cell lysates.
通过利用肽核酸（PNA）模板化，已开发出一种在纳摩尔反应物浓度下运行的快速且具有化学选择性的硒代胱氨酸-硒代酯肽连接反应。对模板化肽连接反应的动力学分析表明，硒代胱氨酸-硒代酯反应比传统的半胱氨酸天然化学连接反应快10倍，据我们所知，这是迄今为止报道的最快的模板化连接反应。通过形成发夹状分子结构以及在一种新型的基于纸的横向流动分析方法中，该技术的效率和操作简便性得到了凸显，该方法可用于快速且序列特异性地检测寡核苷酸，包括细胞裂解物中的微小RNA（miRNA）。
