Department of Oncology, Affiliated Hospital of Qingdao University, Qingdao, China.
Eur Rev Med Pharmacol Sci. 2018 Mar;22(6):1672-1679. doi: 10.26355/eurrev_201803_14579.
To explore the association between miR-1298 expression and clinicopathological factors, prognosis of gastric cancer (GC) patients and biological functions underlying the GC progression.
Expression of miR-1298 was examined by qRT-PCR in GC tissues and cells, the adjacent normal tissues and normal gastric cell line GES-1 cells were used as controls. Association of disease-free survival (DFS) and overall survival (OS) time with miR-1298 expression was analyzed by Kaplan-Meier analysis and log-rank test. Univariate and multivariate analysis were also performed to analyze relative prognostic risk factors of GC patients. Cell proliferation and invasion assays were used to examine cell proliferation and invasion capacities in vitro. The relative protein expression was analyzed by Western blot analysis.
MiR-1298 expression was lower in GC tissues and cells, compared to adjacent normal tissues and GES-1 cells, respectively. Lower miR-1298 expression levels were associated with lymph node metastasis and TNM stage. Kaplan-Meier analysis showed that lower miR-1298 expression predicted poor DFS and OS of GC patients. Furthermore, we demonstrated that lymph node metastasis, TNM stage, and lower miR-1298 expression were independent risk factors for DFS and OS in GC patients. In vitro, miR-1298 overexpression inhibited cell proliferation and invasion abilities. Additionally, our results revealed that miR-1298 overexpression suppressed PI3K/AKT signaling pathway in GC cells.
Our evidence indicated that miR-1298 may provide a specifically promising target for therapy of GC patients.
探讨 miR-1298 表达与胃癌（GC）患者临床病理因素、预后的关系及其在 GC 进展中的生物学功能。
采用 qRT-PCR 检测 GC 组织和细胞中 miR-1298 的表达，以相邻正常组织和正常胃细胞系 GES-1 细胞作为对照。采用 Kaplan-Meier 分析和对数秩检验分析 miR-1298 表达与无病生存（DFS）和总生存（OS）时间的关系。采用单因素和多因素分析分析 GC 患者的相对预后危险因素。采用细胞增殖和侵袭实验检测体外细胞增殖和侵袭能力。采用 Western blot 分析检测相对蛋白表达。
与相邻正常组织和 GES-1 细胞相比，GC 组织和细胞中 miR-1298 的表达水平较低。miR-1298 表达水平较低与淋巴结转移和 TNM 分期有关。Kaplan-Meier 分析表明，miR-1298 表达水平较低预示 GC 患者DFS 和 OS 较差。此外，我们还表明，淋巴结转移、TNM 分期和 miR-1298 表达水平降低是 GC 患者 DFS 和 OS 的独立危险因素。在体外，miR-1298 过表达抑制了细胞增殖和侵袭能力。此外，我们的结果表明，miR-1298 过表达抑制了 GC 细胞中的 PI3K/AKT 信号通路。
我们的研究结果表明，miR-1298 可能为 GC 患者的治疗提供一个有前景的特异性靶点。
