Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan Hubei Province, China.
Department of General Practice, Renmin Hospital of Wuhan University, Wuhan Hubei Province, China.
Bioengineered. 2021 Dec;12(1):1150-1160. doi: 10.1080/21655979.2021.1909951.
The dysregulation of microRNAs (miRNAs) expression is relevant to the progression of many tumors. As reported, the abnormal expression of miR-1269b is pivotal in certain cancers' progression. This work was designed to study the role and hidden mechanism of miR-1269b in gastric cancer (GC) progression. In this work, we proved that miR-1269b was lowly expressed in GC tissues and cell lines, which was associated with larger tumor size and lymph node metastasis. MiR-1269b overexpression repressed the multiplication, migration and invasion of GC cells while miR-1269b inhibition had the opposite effects. Methyltransferase-like 3 (METTL3) was identified as the direct target of miR-1269b in GC cells, and its overexpression reversed the inhibitory effect of transfection of miR-1269b mimics on GC cell viability, migration and invasion. On all accounts, these data indicated that miR-1269b inhibits GC progression via targeting METTL3.
miRNAs 表达失调与许多肿瘤的进展有关。据报道，miR-1269b 的异常表达在某些癌症的进展中起着关键作用。本研究旨在探讨 miR-1269b 在胃癌（GC）进展中的作用和潜在机制。本研究证明，miR-1269b 在 GC 组织和细胞系中低表达，与肿瘤体积较大和淋巴结转移有关。miR-1269b 过表达抑制 GC 细胞的增殖、迁移和侵袭，而 miR-1269b 抑制则产生相反的效果。甲基转移酶样 3（METTL3）被鉴定为 GC 细胞中 miR-1269b 的直接靶标，其过表达逆转了 miR-1269b 模拟物转染对 GC 细胞活力、迁移和侵袭的抑制作用。综上所述，这些数据表明 miR-1269b 通过靶向 METTL3 抑制 GC 进展。
