Vedeler C A, Matre R
Broegelntann Research Laboratory for Microbiology, Gade Institute, University of Bergen, Norway.
J Neuroimmunol. 1988 Mar;17(4):315-22. doi: 10.1016/0165-5728(88)90122-1.
Receptors for C3 and C4 in human peripheral nerve tissue were studied by examining the adherence of complement (C) coated erythrocytes (E) and by using monoclonal antibodies against epitopes on the receptors for C3b (CR1), C3d (CR2) and C3bi (CR3). E (erythrocyte)-bearing C3b or C4b adhered to sections of myelinated peripheral nerves and the binding was inhibited only by anti-CR1 antibodies. By immunofluorescence, anti-CR1 antibodies stained the nerve fibres, whereas anti-CR2 and anti-CR3 antibodies did not. The staining was apparently localized to the Schwann cell membrane. E-bearing C3bi or C3d did not adhere to myelinated or unmyelinated nerves. CR1 are therefore the only C3 receptors expressed in human peripheral nerves. E-bearing C3b or C4b did not adhere to unmyelinated nerves from adults or to nerves from fetuses at a gestational age of approximately 21 weeks, whereas monoclonal anti-CR1 antibodies stained myelinated, unmyelinated and fetal nerves equally well. The results indicate that CR1 in unmyelinated and fetal nerves are either functionally inactive or express a lower affinity for C3b/C4b than CR1 in myelinated nerves. There were no significant differences in the binding of E-bearing C3b or C4b to myelinated peripheral nerves from 50 individuals, indicating that CR1 activity is not distributed phenotypically.
通过检测补体（C）包被红细胞（E）的黏附情况，并使用针对C3b（CR1）、C3d（CR2）和C3bi（CR3）受体表位的单克隆抗体，对人周围神经组织中的C3和C4受体进行了研究。携带C3b或C4b的E黏附于有髓周围神经切片，且这种结合仅被抗CR1抗体抑制。通过免疫荧光法，抗CR1抗体可使神经纤维染色，而抗CR2和抗CR3抗体则不能。染色明显定位于施万细胞膜。携带C3bi或C3d的E不黏附于有髓或无髓神经。因此，CR1是人类周围神经中唯一表达的C3受体。携带C3b或C4b的E不黏附于成人的无髓神经或妊娠约21周胎儿的神经，而单克隆抗CR1抗体对有髓、无髓和胎儿神经的染色效果相同。结果表明，无髓神经和胎儿神经中的CR1要么功能不活跃，要么对C3b/C4b的亲和力低于有髓神经中的CR1。携带C3b或C4b的E与50名个体的有髓周围神经的结合没有显著差异，表明CR1活性在表型上没有分布差异。
