Department of Thyroid and Breast Surgery, The People's Hospital of Liaoning Province, Shenyang, China.
Eur Rev Med Pharmacol Sci. 2020 Apr;24(8):4361-4367. doi: 10.26355/eurrev_202004_21017.
The aim of this study was to explore the expression of long non-coding ribonucleic acid (lncRNA) FALEC (hereinafter referred to as FALEC) in papillary thyroid carcinoma (PTC) and its effects on the proliferation, invasion, and metastasis of PTC cells.
Quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR) was performed to measure the expression level of FALEC in 48 cases of PTC tissues and cells. The small interfering (si)-FALEC was synthesized and transfected into PTC cells. Interference efficiency was confirmed via qRT-PCR assay. Subsequently, the effect of FALEC on the proliferation of PTC cells was determined by cell counting kit-8 (CCK-8) assay. Wound healing and transwell assays were conducted to detect the effects of FALEC on the invasion, migration, and metastasis of PTC cells. Additionally, changes in the protein expression of Wnt/β-catenin signaling pathway molecular markers was detected via Western blotting.
The expression level of FALEC was significantly higher in PTC tissues than that of adjacent normal tissues. FALEC expression was significantly up-regulated in PTC cell lines, as well. CCK-8 assay revealed that the proliferation ability of PTC cells was remarkably weakened after down-regulation of FALEC in vitro. Wound healing and transwell assays demonstrated that, compared with si-normal control (NC) group, the migration and invasion capabilities declined significantly in si-FALEC group. Furthermore, the Western blotting analysis indicated that the expression of Wnt/β-catenin signaling pathway molecular markers was changed after the interference in FALEC expression.
FALEC expression was up-regulated in PTC tissues and cell lines. Highly expressed FALEC facilitated the proliferation, migration, and invasion of PTC by regulating the Wnt/β-catenin signaling pathway.
本研究旨在探讨长链非编码 RNA（lncRNA）FALEC（以下简称 FALEC）在甲状腺乳头状癌（PTC）中的表达及其对 PTC 细胞增殖、侵袭和转移的影响。
采用实时定量逆转录聚合酶链反应（qRT-PCR）检测 48 例 PTC 组织和细胞中 FALEC 的表达水平。合成小干扰（si）-FALEC 并转染至 PTC 细胞。通过 qRT-PCR 检测证实干扰效率。随后，通过细胞计数试剂盒-8（CCK-8）测定 FALEC 对 PTC 细胞增殖的影响。通过划痕愈合和 Transwell 实验检测 FALEC 对 PTC 细胞侵袭、迁移和转移的影响。此外，通过 Western blot 检测 Wnt/β-catenin 信号通路分子标志物的蛋白表达变化。
FALEC 在 PTC 组织中的表达水平明显高于相邻正常组织。FALEC 在 PTC 细胞系中的表达也显著上调。CCK-8 实验表明，体外下调 FALEC 后 PTC 细胞的增殖能力显著减弱。划痕愈合和 Transwell 实验表明，与 si-NC 组相比，si-FALEC 组的迁移和侵袭能力显著下降。此外，Western blot 分析表明，FALEC 表达干扰后 Wnt/β-catenin 信号通路分子标志物的表达发生变化。
FALEC 在 PTC 组织和细胞系中表达上调。高表达的 FALEC 通过调节 Wnt/β-catenin 信号通路促进 PTC 的增殖、迁移和侵袭。
